Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
15  structures 481  species 2  interactions 811  sequences 17  architectures

Family: Pex14_N (PF04695)

Summary: Peroxisomal membrane anchor protein (Pex14p) conserved region

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "PEX14". More...

PEX14 Edit Wikipedia article

PEX14
Protein PEX14 PDB 2W84.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases PEX14, NAPP2, PBD13A, Pex14p, dJ734G22.2, peroxisomal biogenesis factor 14
External IDs MGI: 1927868 HomoloGene: 37936 GeneCards: PEX14
Genetically Related Diseases
breast cancer[1]
RNA expression pattern
PBB GE PEX14 203503 s at tn.png

PBB GE PEX14 33760 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_004565

NM_019781

RefSeq (protein)

NP_004556

NP_062755.1
NP_062755

Location (UCSC) Chr 1: 10.47 – 10.63 Mb Chr 4: 148.96 – 149.1 Mb
PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse
Peroxisomal membrane anchor protein (Pex14p) conserved region
Identifiers
Symbol Pex14_N
Pfam PF04695
InterPro IPR006785

Peroxisomal membrane protein PEX14 is a protein that in humans is encoded by the PEX14 gene.[4][5]

Function

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.[5]

Interactions

PEX14 has been shown to interact with

References

  1. ^ "Diseases that are genetically associated with PEX14 view/edit references on wikidata". 
  2. ^ "Human PubMed Reference:". 
  3. ^ "Mouse PubMed Reference:". 
  4. ^ Fransen M, Terlecky SR, Subramani S (Jul 1998). "Identification of a human PTS1 receptor docking protein directly required for peroxisomal protein import". Proceedings of the National Academy of Sciences of the United States of America. 95 (14): 8087–92. doi:10.1073/pnas.95.14.8087. PMC 20933Freely accessible. PMID 9653144. 
  5. ^ a b "Entrez Gene: PEX14 peroxisomal biogenesis factor 14". 
  6. ^ a b Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y (Mar 2002). "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import". Molecular and Cellular Biology. 22 (6): 1639–55. doi:10.1128/MCB.22.6.1639-1655.2002. PMC 135613Freely accessible. PMID 11865044. 
  7. ^ Okumoto K, Abe I, Fujiki Y (Aug 2000). "Molecular anatomy of the peroxin Pex12p: ring finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a ring peroxin, Pex10p". The Journal of Biological Chemistry. 275 (33): 25700–10. doi:10.1074/jbc.M003303200. PMID 10837480. 
  8. ^ Shimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y (Apr 1999). "The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". The Journal of Biological Chemistry. 274 (18): 12593–604. doi:10.1074/jbc.274.18.12593. PMID 10212238. 
  9. ^ a b Managadze D, Würtz C, Sichting M, Niehaus G, Veenhuis M, Rottensteiner H (Jun 2007). "The peroxin PEX14 of Neurospora crassa is essential for the biogenesis of both glyoxysomes and Woronin bodies". Traffic. 8 (6): 687–701. doi:10.1111/j.1600-0854.2007.00560.x. PMID 17461798. 

Further reading

External links

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Peroxisomal membrane anchor protein (Pex14p) conserved region Provide feedback

Family of peroxisomal membrane anchor proteins which bind the PTS1 (peroxisomal targeting signal) receptor and are required for the import of PTS1-containing proteins into peroxisomes. Loss of functional Pex14p results in defects in both the PTS1 and PTS2-dependent import pathways. Deletion analysis of this conserved region implicates it in selective peroxisome degradation. In the majority of members this region is situated at the N-terminus of the protein [1,2].

Literature references

  1. Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel JA, Veenhuis M, Kunau WH; , Cell 1997;89:83-92.: Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. PUBMED:9094717 EPMC:9094717

  2. Bellu AR, Komori M, van der Klei IJ, Kiel JA, Veenhuis M; , J Biol Chem 2001;276:44570-44574.: Peroxisome biogenesis and selective degradation converge at Pex14p. PUBMED:11564741 EPMC:11564741


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006785

This conserved region defines a group of peroxisomal membrane anchor proteins which bind the PTS1 (peroxisomal targeting signal) receptor and are required for the import of PTS1-containing proteins into peroxisomes. Loss of functional Pex14p results in defects in both the PTS1 and PTS2-dependent import pathways. Deletion analysis of this conserved region implicates it in selective peroxisome degradation. In the majority of members this region is situated at the N terminus of the protein [PUBMED:9094717, PUBMED:11564741].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(144)
Full
(811)
Representative proteomes UniProt
(1450)
NCBI
(1564)
Meta
(1)
RP15
(159)
RP35
(389)
RP55
(588)
RP75
(761)
Jalview View  View  View  View  View  View  View  View  View 
HTML View  View               
PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(144)
Full
(811)
Representative proteomes UniProt
(1450)
NCBI
(1564)
Meta
(1)
RP15
(159)
RP35
(389)
RP55
(588)
RP75
(761)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(144)
Full
(811)
Representative proteomes UniProt
(1450)
NCBI
(1564)
Meta
(1)
RP15
(159)
RP35
(389)
RP55
(588)
RP75
(761)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_4121 (release 7.5)
Previous IDs: none
Type: Family
Author: Waterfield DI, Finn RD
Number in seed: 144
Number in full: 811
Average length of the domain: 122.90 aa
Average identity of full alignment: 26 %
Average coverage of the sequence by the domain: 31.74 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 17690987 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.8 26.8
Trusted cut-off 26.8 26.8
Noise cut-off 26.7 26.7
Model length: 151
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Interactions

There are 2 interactions for this family. More...

Pex14_N Pex19

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Pex14_N domain has been found. There are 15 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

Loading structure mapping...