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0  structures 117  species 0  interactions 136  sequences 4  architectures

Family: Spo12 (PF05032)

Summary: Spo12 family

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Spo12 family Provide feedback

This family of proteins includes Spo12 from S. cerevisiae P17123. The Spo12 protein plays a regulatory role in two of the most fundamental processes of biology, mitosis and meiosis, and yet its biochemical function remains elusive [1]. Spo12 is a nuclear protein [2]. Spo12 is a component of the FEAR (Cdc fourteen early anaphase release) regulatory network, that promotes Cdc14 release from the nucleolus during early anaphase [3]. The FEAR network is comprised of the polo kinase Cdc5, the separase Esp1, the kinetochore-associated protein Slk19, and Spo12 [3].

Literature references

  1. Shah R, Jensen S, Frenz LM, Johnson AL, Johnston LH; , Genetics 2001;159:965-980.: The Spo12 protein of Saccharomyces cerevisiae: a regulator of mitotic exit whose cell cycle-dependent degradation is mediated by the anaphase-promoting complex. PUBMED:11729145 EPMC:11729145

  2. Chaves SR, Blobel G; , J Biol Chem 2001;276:17712-17717.: Nuclear import of Spo12p, a protein essential for meiosis. PUBMED:11278742 EPMC:11278742

  3. Stegmeier F, Visintin R, Amon A; , Cell 2002;108:207-220.: Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. PUBMED:11832211 EPMC:11832211


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007727

This family of proteins includes Spo12 from Saccharomyces cerevisiae SWISSPROT. The Spo12 protein plays a regulatory role in two of the most fundamental processes of biology, mitosis and meiosis, and yet its biochemical function remains elusive [PUBMED:11729145]. Spo12 is a nuclear protein [PUBMED:11278742]. Spo12 is a component of the FEAR (Cdc fourteen early anaphase release) regulatory network, which promotes Cdc14 release from the nucleolus during early anaphase [PUBMED:11832211]. The FEAR network is comprised of the polo kinase Cdc5, the separase Esp1, the kinetochore-associated protein Slk19, and Spo12 [PUBMED:11832211].

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(9)
Full
(136)
Representative proteomes NCBI
(116)
Meta
(0)
RP15
(27)
RP35
(53)
RP55
(81)
RP75
(96)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(9)
Full
(136)
Representative proteomes NCBI
(116)
Meta
(0)
RP15
(27)
RP35
(53)
RP55
(81)
RP75
(96)
Alignment:
Format:
Order:
Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(9)
Full
(136)
Representative proteomes NCBI
(116)
Meta
(0)
RP15
(27)
RP35
(53)
RP55
(81)
RP75
(96)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_51047 (release 7.6)
Previous IDs: none
Type: Family
Author: Wood V
Number in seed: 9
Number in full: 136
Average length of the domain: 34.20 aa
Average identity of full alignment: 45 %
Average coverage of the sequence by the domain: 21.64 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.5 20.5
Trusted cut-off 21.1 21.3
Noise cut-off 20.4 20.4
Model length: 35
Family (HMM) version: 7
Download: download the raw HMM for this family

Species distribution

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