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8  structures 836  species 0  interactions 1539  sequences 21  architectures

Family: LANC_like (PF05147)

Summary: Lanthionine synthetase C-like protein

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Lanthionine synthetase C-like protein Provide feedback

Lanthionines are thioether bridges that are putatively generated by dehydration of Ser and Thr residues followed by addition of cysteine residues within the peptide. This family contains the lanthionine synthetase C-like proteins 1 and 2 which are related to the bacterial lanthionine synthetase components C (LanC). LANCL1 (P40 seven-transmembrane-domain protein) and LANCL2 (testes-specific adriamycin sensitivity protein) are thought to be peptide-modifying enzyme components in eukaryotic cells. Both proteins are produced in large quantities in the brain and testes and may have role in the immune surveillance of these organs [1]. Lanthionines are found in lantibiotics, which are peptide-derived, post-translationally modified antimicrobials produced by several bacterial strains [2]. This region contains seven internal repeats.

Literature references

  1. Mayer H, Bauer H, Breuss J, Ziegler S, Prohaska R; , Gene 2001;269:73-80.: Characterization of rat LANCL1, a novel member of the lanthionine synthetase C-like protein family, highly expressed in testis and brain. PUBMED:11376939 EPMC:11376939

  2. Xie L, Chatterjee C, Balsara R, Okeley NM, van der Donk WA; , Biochem Biophys Res Commun 2002;295:952-957.: Heterologous expression and purification of SpaB involved in subtilin biosynthesis. PUBMED:12127987 EPMC:12127987


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007822

The LanC-like protein superfamily encompasses a highly divergent group of peptide-modifying enzymes, including the eukaryotic and bacterial lanthionine synthetase C-like proteins (LanC) [PUBMED:11474189, PUBMED:10944443, PUBMED:12566319]; subtilin biosynthesis protein SpaC from Bacillus subtilis [PUBMED:1735728, PUBMED:1539969]; epidermin biosynthesis protein EpiC from Staphylococcus epidermidis [PUBMED:1740156]; nisin biosynthesis protein NisC from Lactococcus lactis [PUBMED:8161176, PUBMED:7689965, PUBMED:1482192]; GCR2 from Arabidopsis thaliana [PUBMED:17347412]; and many others.

The 3D structure of the lantibiotic cyclase from L. lactis has been determined by X-ray crystallography to 2.5A resolution [PUBMED:16527981]. The globular structure is characterised by an all-alpha fold, in which an outer ring of helices envelops an inner toroid composed of 7 shorter, hydrophobic helices. This 7-fold hyrophobic periodicity has led several authors to claim various members of the family, including eukaryotic LanC-1 and GCR2, to be novel G protein-coupled receptors [PUBMED:17347412, PUBMED:9512664]; some of these claims have since been corrected [PUBMED:10944443, PUBMED:18086512, PUBMED:17894782].

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(47)
Full
(1539)
Representative proteomes NCBI
(1232)
Meta
(30)
RP15
(162)
RP35
(275)
RP55
(398)
RP75
(490)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Format an alignment

  Seed
(47)
Full
(1539)
Representative proteomes NCBI
(1232)
Meta
(30)
RP15
(162)
RP35
(275)
RP55
(398)
RP75
(490)
Alignment:
Format:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(47)
Full
(1539)
Representative proteomes NCBI
(1232)
Meta
(30)
RP15
(162)
RP35
(275)
RP55
(398)
RP75
(490)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_6095 (release 7.7)
Previous IDs: none
Type: Family
Author: Moxon SJ, Finn RD, Fenech M
Number in seed: 47
Number in full: 1539
Average length of the domain: 282.40 aa
Average identity of full alignment: 16 %
Average coverage of the sequence by the domain: 54.42 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.2 23.2
Trusted cut-off 23.2 23.2
Noise cut-off 23.1 23.1
Model length: 355
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the LANC_like domain has been found. There are 8 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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