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1  structure 293  species 0  interactions 336  sequences 2  architectures

Family: CENP-N (PF05238)

Summary: Kinetochore protein CHL4 like

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Kinetochore protein CHL4 like Provide feedback

CHL4 is a protein involved in chromosome segregation [1]. It is a component of the central kinetochore which mediates the attachment of the centromere to the mitotic spindle [2]. CENP-N is one of the components that assembles onto the CENP-A-nucleosome-associated (NAC) centromere. The centromere, which is the basic element of chromosome inheritance, is epigenetically determined in mammals. CENP-A, the centromere-specific histone H3 variant, assembles an array of nucleosomes and it is this that seems to be the prime candidate for specifying centromere identity. CENP-A nucleosomes directly recruit a proximal CENP-A nucleosome associated complex (NAC) comprised of CENP-M, CENP-N and CENP-T, CENP-U(50), CENP-C and CENP-H. Assembly of the CENP-A NAC at centromeres is dependent on CENP-M, CENP-N and CENP-T. Additionally, there are seven other subunits which make up the CENP-A-nucleosome distal (CAD) centromere, CENP-K, CENP-L, CENP-O, CENP-P, CENP-Q, CENP-R and CENP-S, also assembling on the CENP-A NAC [4].

Literature references

  1. Kouprina N, Kirillov A, Kroll E, Koryabin M, Shestopalov B, Bannikov V, Zakharyev V, Larionov V; , Genetics 1993;135:327-341.: Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast. PUBMED:8243998 EPMC:8243998

  2. Pot I, Measday V, Snydsman B, Cagney G, Fields S, Davis TN, Muller EG, Hieter P; , Mol Biol Cell. 2003;14:460-476.: Chl4p and iml3p are two new members of the budding yeast outer kinetochore. PUBMED:12589047 EPMC:12589047

  3. Meraldi P, McAinsh AD, Rheinbay E, Sorger PK; , Genome Biol. 2006;7:R23.: Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins. PUBMED:16563186 EPMC:16563186

  4. Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW; , Nat Cell Biol. 2006;8:458-469.: The human CENP-A centromeric nucleosome-associated complex. PUBMED:16622419 EPMC:16622419


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007902

This family includes Chl4 from budding yeasts, mis15 from fission yeasts and centromere protein N (CENP-N) from animals.

In Saccharomyces cerevisiae, Chl4 is an outer kinetochore structural component required for chromosome stability [PUBMED:12589047]. Chl4 is a component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p [PUBMED:12589047]. It is required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation [PUBMED:9339342].

In Schizosaccharomyces pombe, mis15 is a subunit of the Sim4 complex, which is required for loading the DASH complex onto the kinetochore via interaction with dad1 [PUBMED:21445296]. It is required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialised chromatin at the centromere [PUBMED:16855021].

In humans, centromere protein N (CENP-N) is a component of the CENPA-NAC (nucleosome-associated) complex, which plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation [PUBMED:16716197]. CENP-N localises exclusively in the kinetochore domain of centromeres [PUBMED:16622419]. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate [PUBMED:19543270].

Gene Ontology

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Domain organisation

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Alignments

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RP35
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RP55
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RP75
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  Seed
(28)
Full
(336)
Representative proteomes NCBI
(517)
Meta
(0)
RP15
(28)
RP35
(89)
RP55
(149)
RP75
(214)
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  Seed
(28)
Full
(336)
Representative proteomes NCBI
(517)
Meta
(0)
RP15
(28)
RP35
(89)
RP55
(149)
RP75
(214)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

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Curation and family details

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Curation View help on the curation process

Seed source: Wood V
Previous IDs: CHL4;
Type: Family
Author: Wood V
Number in seed: 28
Number in full: 336
Average length of the domain: 383.60 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 92.72 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 26.2 25.4
Noise cut-off 19.6 21.9
Model length: 416
Family (HMM) version: 9
Download: download the raw HMM for this family

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CENP-N domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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