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10  structures 763  species 0  interactions 3346  sequences 78  architectures

Family: Glyco_transf_34 (PF05637)

Summary: galactosyl transferase GMA12/MNN10 family

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

galactosyl transferase GMA12/MNN10 family Provide feedback

This family contains a number of glycosyltransferase enzymes that contain a DXD motif. This family includes a number of C. elegans homologues where the DXD is replaced by DXH. Some members of this family are included in glycosyltransferase family 34.

Literature references

  1. Chappell TG, Hajibagheri MA, Ayscough K, Pierce M, Warren G; , Mol Biol Cell 1994;5:519-528.: Localization of an alpha 1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe. PUBMED:7522655 EPMC:7522655

  2. Yoko-o T, Roy SK, Jigami Y; , Eur J Biochem 1998;257:630-637.: Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe. PUBMED:9839953 EPMC:9839953

  3. Dean N, Poster JB; , Glycobiology 1996;6:73-81.: Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases. PUBMED:8991513 EPMC:8991513


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR008630

This family represents a group of glycosyltransferases from plants and fungi, including Mnn10/Mnn11 from budding yeasts, Gma12/Gmh1-3 from fission yeasts and xyloglucan 6-xylosyltransferase 1/2 (XXT1/XXT2) from Arabidopsis.

Mnn10 and Mnn11 are subunits of a Golgi mannosyltransferase complex, which mediates elongation of the polysaccharide mannan backbone [ PUBMED:10037752 ].

Gma12 is involved in the O- and N-linked oligosaccharide modification of proteins transported through the Golgi stack, while Gmh3 is involved in the galactosylation of the N-linked core oligosaccharide Man9GlcNAc2 [ PUBMED:9839953 ].

XXT1 and XXT2 are xylosyltransferases specific to UDP-D-xylose that accepts both cellopentaose and cellohexaose as substrates, with a better use of cellohexaose, to produce xyloglucan [ PUBMED:18544630 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(14)
Full
(3346)
Representative proteomes UniProt
(7131)
RP15
(570)
RP35
(1627)
RP55
(2606)
RP75
(3583)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(14)
Full
(3346)
Representative proteomes UniProt
(7131)
RP15
(570)
RP35
(1627)
RP55
(2606)
RP75
(3583)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(14)
Full
(3346)
Representative proteomes UniProt
(7131)
RP15
(570)
RP35
(1627)
RP55
(2606)
RP75
(3583)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_6000 (release 8.0)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Wood V
Number in seed: 14
Number in full: 3346
Average length of the domain: 210.50 aa
Average identity of full alignment: 24 %
Average coverage of the sequence by the domain: 52.14 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.0 21.0
Trusted cut-off 21.0 21.0
Noise cut-off 20.9 20.9
Model length: 239
Family (HMM) version: 14
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_transf_34 domain has been found. There are 10 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0X578 View 3D Structure Click here
A0A0P0X578 View 3D Structure Click here
A0A0R0KTK1 View 3D Structure Click here
A0A1D6EKW1 View 3D Structure Click here
A0A1D6H9U5 View 3D Structure Click here
A0A1D6IT87 View 3D Structure Click here
A0A1D6JAC2 View 3D Structure Click here
A0A1D6JAC2 View 3D Structure Click here
A0A1D6JBS3 View 3D Structure Click here
A0A1D6K093 View 3D Structure Click here
A0A1D6LIU8 View 3D Structure Click here
A0A1D6LIU8 View 3D Structure Click here
A0A1D6ML09 View 3D Structure Click here
A0A1D6N847 View 3D Structure Click here
A0A1D6PC37 View 3D Structure Click here
A0A1D6PC42 View 3D Structure Click here
A0A1D6PC47 View 3D Structure Click here
A0A1D6Q5X3 View 3D Structure Click here
A0A1D8PCR3 View 3D Structure Click here
C0P7T0 View 3D Structure Click here
C7J0P3 View 3D Structure Click here
I1J9F9 View 3D Structure Click here
I1JZM5 View 3D Structure Click here
I1K2F3 View 3D Structure Click here
I1L100 View 3D Structure Click here
I1LH49 View 3D Structure Click here
I1MGL4 View 3D Structure Click here
I1MTW4 View 3D Structure Click here
I1NB50 View 3D Structure Click here
K7TEK8 View 3D Structure Click here
K7TWY3 View 3D Structure Click here
K7U776 View 3D Structure Click here
K7UBB6 View 3D Structure Click here
K7UBP6 View 3D Structure Click here
K7UMG2 View 3D Structure Click here
K7VMJ7 View 3D Structure Click here
K7W0A9 View 3D Structure Click here
O13640 View 3D Structure Click here
O22775 View 3D Structure Click here
O81007 View 3D Structure Click here
O94622 View 3D Structure Click here
P46985 View 3D Structure Click here
P50108 View 3D Structure Click here
P78817 View 3D Structure Click here
Q09174 View 3D Structure Click here
Q09679 View 3D Structure Click here
Q09681 View 3D Structure Click here
Q10359 View 3D Structure Click here
Q10MK2 View 3D Structure Click here
Q10MQ0 View 3D Structure Click here
Q2QXP0 View 3D Structure Click here
Q2R2W8 View 3D Structure Click here
Q59UR2 View 3D Structure Click here
Q5TIM9 View 3D Structure Click here
Q6H765 View 3D Structure Click here
Q6Z5M3 View 3D Structure Click here
Q9CA75 View 3D Structure Click here
Q9LF80 View 3D Structure Click here
Q9LZJ3 View 3D Structure Click here
Q9M9U0 View 3D Structure Click here
Q9SVF4 View 3D Structure Click here
Q9SZG1 View 3D Structure Click here