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0  structures 382  species 0  interactions 405  sequences 6  architectures

Family: CENP-H (PF05837)

Summary: Centromere protein H (CENP-H)

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This is the Wikipedia entry entitled "CENPH". More...

CENPH Edit Wikipedia article

CENPH
Identifiers
AliasesCENPH, centromere protein H
External IDsOMIM: 605607 MGI: 1349448 HomoloGene: 32519 GeneCards: CENPH
Gene location (Human)
Chromosome 5 (human)
Chr.Chromosome 5 (human)[1]
Chromosome 5 (human)
Genomic location for CENPH
Genomic location for CENPH
Band5q13.2Start69,189,574 bp[1]
End69,210,357 bp[1]
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_022909

NM_021886

RefSeq (protein)

NP_075060

NP_068686

Location (UCSC)Chr 5: 69.19 – 69.21 MbChr 13: 100.76 – 100.78 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
CENP-H
Identifiers
SymbolCENP-H
PfamPF05837
InterProIPR008426

Centromere protein H is a protein that in humans is encoded by the CENPH gene.[5][6][7]

Function

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. CENP-H is required for the localisation of CENP-C, but not CENP-A, to the centromere. However, it may be involved in the incorporation of newly synthesised CENP-A into centromeres via its interaction with the CENP-A/CENP-HI complex.[8] CENP-H localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.[9] CENP-H contains a coiled-coil structure and a nuclear localisation signal.[9]

Studies show that CENP-H may be associated with certain human cancers.[10][11]

CENP-H shows sequence similarity to the Schizosaccharomyces pombe kinetochore protein Fta3 which is a subunit of the Sim4 complex. This complex is required for loading the DASH complex onto the kinetochore via interaction with dad1. Fta2, Fta3 and Fta4 associate with the central core and inner repeat region of the centromere.[12]

Other Protein Interactions

CENPH has also been shown to interact with KIAA0090.[13] The significance of this interaction is unclear.

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000153044 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000045273 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Sugata N, Li S, Earnshaw WC, Yen TJ, Yoda K, Masumoto H, Munekata E, Warburton PE, Todokoro K (Jan 2001). "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes". Hum Mol Genet. 9 (19): 2919–26. doi:10.1093/hmg/9.19.2919. PMID 11092768.
  6. ^ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat Cell Biol. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
  7. ^ "Entrez Gene: CENPH centromere protein H".
  8. ^ Fukagawa, T.; Mikami, Y.; Nishihashi, A.; Regnier, V.; Haraguchi, T.; Hiraoka, Y.; Sugata, N.; Todokoro, K.; Brown, W.; Ikemura, T. (2001). "CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells". The EMBO Journal. 20 (16): 4603–4617. doi:10.1093/emboj/20.16.4603. PMC 125570. PMID 11500386.
  9. ^ a b Sugata N, Munekata E, Todokoro K (September 1999). "Characterization of a novel kinetochore protein, CENP-H". J. Biol. Chem. 274 (39): 27343–6. doi:10.1074/jbc.274.39.27343. PMID 10488063.
  10. ^ Guo XZ, Zhang G, Wang JY, Liu WL, Wang F, Dong JQ, Xu LH, Cao JY, Song LB, Zeng MS (2008). "Prognostic relevance of Centromere protein H expression in esophageal carcinoma". BMC Cancer. 8: 233. doi:10.1186/1471-2407-8-233. PMC 2535782. PMID 18700042.
  11. ^ Liao WT, Song LB, Zhang HZ, Zhang X, Zhang L, Liu WL, Feng Y, Guo BH, Mai HQ, Cao SM, Li MZ, Qin HD, Zeng YX, Zeng MS (January 2007). "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival". Clin. Cancer Res. 13 (2 Pt 1): 508–14. doi:10.1158/1078-0432.CCR-06-1512. PMID 17255272.
  12. ^ Liu X, McLeod I, Anderson S, Yates JR, He X (August 2005). "Molecular analysis of kinetochore architecture in fission yeast". EMBO J. 24 (16): 2919–30. doi:10.1038/sj.emboj.7600762. PMC 1187945. PMID 16079914.
  13. ^ Prieto C, De Las Rivas J (July 2006). "APID: Agile Protein Interaction DataAnalyzer". Nucleic Acids Res. 34 (Web Server issue): W298–302. doi:10.1093/nar/gkl128. PMC 1538863. PMID 16845013. Archived from the original on 2010-04-09.

External links

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR008426


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Centromere protein H (CENP-H) Provide feedback

This family consists of several eukaryotic centromere protein H (CENP-H) sequences. Macromolecular centromere-kinetochore complex plays a critical role in sister chromatid separation, but its complete protein composition as well as its precise dynamic function during mitosis has not yet been clearly determined. CENP-H contains a coiled-coil structure and a nuclear localisation signal. CENP-H is specifically and constitutively localised in kinetochores throughout the cell cycle. CENP-H may play a role in kinetochore organisation and function throughout the cell cycle [1]. This the C-terminus of the region, which is conserved from fungi to humans.

Literature references

  1. Sugata N, Munekata E, Todokoro K; , J Biol Chem 1999;274:27343-27346.: Characterization of a novel kinetochore protein, CENP-H. PUBMED:10488063 EPMC:10488063

  2. Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi T, Hiraoka Y, Sugata N, Todokoro K, Brown W, Ikemura T; , EMBO J 2001;20:4603-4617.: CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells. PUBMED:11500386 EPMC:11500386


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR008426

Chromosome segregation in eukaryotes requires the kinetochore, a multi-protein structure that assembles on centromeric DNA, and which acts to link chromosomes to spindle microtubules. Kinetochore structure and composition is highly conserved among vertebrates. The inner kinetochore is essential for kinetochore assembly, and is involved in chromosome segregation via regulation of the spindle. Inner kinetochore components include the multi-subunit CENP-H/I complex, which may function, in part, in directing centromere protein A (CENP-A) deposition to centromeres, where CENP-A is a centromere-specific histone H3 variant required for the organisation of centromeric chromatin during interphase. The CENP-H/I complex contains three functional classes of proteins [PUBMED:16622420, PUBMED:18094054]:

  • CENP-H class (includes CENP-H, -I, -K, -L)
  • CENP-M class (includes CENP-M)
  • CENP-O class (includes CENP-O, -P, -Q, -R, -50)

CENP-H is required for the localisation of CENP-C, but not CENP-A, to the centromere. However, it may be involved in the incorporation of newly synthesised CENP-A into centromeres via its interaction with the CENP-A/CENP-HI complex. CENP-H contains a coiled-coil structure and a nuclear localisation signal. CENP-H is specifically and constitutively localised in kinetochores throughout the cell cycle, and may play a role in kinetochore organisation and function throughout the cell cycle [PUBMED:10488063].

Studies show that CENP-H may be associated with certain human cancers [PUBMED:18700042, PUBMED:17255272].

This entry also includes Schizosaccharomyces pombe Kinetochore protein Fta3, which is a subunit of the Sim4 complex. This complex is required for loading the DASH complex onto the kinetochore via interaction with dad1. Fta2, Fta3 and Fta4 associate with the central core and inner repeat region of the centromere [PUBMED:16079914].

Gene Ontology

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Domain organisation

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(80)
Full
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Representative proteomes UniProt
(571)
NCBI
(773)
Meta
(0)
RP15
(76)
RP35
(181)
RP55
(291)
RP75
(404)
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  Seed
(80)
Full
(405)
Representative proteomes UniProt
(571)
NCBI
(773)
Meta
(0)
RP15
(76)
RP35
(181)
RP55
(291)
RP75
(404)
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Curation View help on the curation process

Seed source: Pfam-B_8705 (release 8.0)
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Moxon SJ
Number in seed: 80
Number in full: 405
Average length of the domain: 115.30 aa
Average identity of full alignment: 27 %
Average coverage of the sequence by the domain: 46.78 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.7 25.7
Trusted cut-off 25.7 25.7
Noise cut-off 25.3 25.6
Model length: 112
Family (HMM) version: 12
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