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14  structures 2225  species 1  interaction 5431  sequences 20  architectures

Family: Arylsulfotrans (PF05935)

Summary: Arylsulfotransferase (ASST)

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Aryl sulfotransferase". More...

Aryl sulfotransferase Edit Wikipedia article

aryl sulfotransferase
EC number
CAS number 9026-09-9
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO
Symbol Arylsulfotransferase
Pfam PF05935
InterPro IPR010262

An aryl sulfotransferase (EC is an enzyme that transfers a sulfate group from phenolic sulfate esters to a phenolic acceptor substrate.[1]

3'-phosphoadenylyl sulfate + a phenol \rightleftharpoons adenosine 3',5'-bisphosphate + an aryl sulfate

Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and phenol, whereas its two products are adenosine 3',5'-bisphosphate and aryl sulfate.

These enzymes are transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:phenol sulfotransferase. Other names in common use include phenol sulfotransferase, sulfokinase, 1-naphthol phenol sulfotransferase, 2-naphtholsulfotransferase, 4-nitrocatechol sulfokinase, arylsulfotransferase, dopamine sulfotransferase, p-nitrophenol sulfotransferase, phenol sulfokinase, ritodrine sulfotransferase, and PST. This enzyme participates in sulfur metabolism.

Structural studies

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1LS6, 1Z28, 1Z29, 2A3R, and 2D06.


  1. ^ Kim SK, Baek MC, Kim DH, Kim BK, Choi EC (1996). "Cloning and sequencing of the Klebsiella K-36 astA gene, encoding an arylsulfate sulfotransferase". Microbiol. Immunol. 40 (8): 531–537. doi:10.1111/j.1348-0421.1996.tb01105.x. PMID 8887346. 

Further reading

  • Romain Y, Demassieux S, Carriere S (1982). "Partial purification and characterization of two isoenzymes involved in the sulfurylation of catecholamines". Biochem. Biophys. Res. Commun. 106 (3): 999–1005. doi:10.1016/0006-291X(82)91810-1. PMID 6956338. 
  • Sekura RD, Jakoby WB (1979). "Phenol sulfotransferases". J. Biol. Chem. 254 (13): 5658–63. PMID 447677. 

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Arylsulfotransferase (ASST) Provide feedback

This family consists of several bacterial Arylsulfotransferase proteins. Arylsulfotransferase (ASST) transfers a sulfate group from phenolic sulfate esters to a phenolic acceptor substrate [1].

Literature references

  1. Baek MC, Kim SK, Kim DH, Kim BK, Choi EC; , Microbiol Immunol 1996;40:531-537.: Cloning and sequencing of the Klebsiella K-36 astA gene, encoding an arylsulfate sulfotransferase. PUBMED:8887346 EPMC:8887346

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR010262

This family consists of several bacterial arylsulphotransferase proteins. Arylsulphotransferase (ASST) transfers a sulphate group from phenolic sulphate esters to a phenolic acceptor substrate [PUBMED:8887346].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Beta_propeller (CL0186), which has the following description:

This large clan contains proteins that contain beta propellers. These are composed of between 6 and 8 repeats. The individual repeats are composed of a four stranded sheet. The clan includes families such as WD40 Pfam:PF00400 where the individual repeats are modeled. The clan also includes families where the entire propeller is modeled such as Pfam:PF02239 usually because the individual repeats are not discernible. These proteins carry out a very wide diversity of functions including catalysis.

The clan contains the following 67 members:

ANAPC4_WD40 Arylesterase Arylsulfotran_2 Arylsulfotrans BBS2_Mid Beta_propel Coatomer_WDAD CPSF_A Cytochrom_D1 DPPIV_N DUF1513 DUF1668 DUF2415 DUF3312 DUF4221 DUF5050 DUF839 eIF2A FG-GAP FG-GAP_2 Ge1_WD40 Glu_cyclase_2 Gmad1 GSDH IKI3 Itfg2 Kelch_1 Kelch_2 Kelch_3 Kelch_4 Kelch_5 Kelch_6 Lactonase Ldl_recept_b Lgl_C LVIVD Me-amine-dh_H MRJP Nbas_N Neisseria_PilC NHL Nucleoporin_N Nup160 PALB2_WD40 PD40 Pectate_lyase22 Peptidase_S9_N Phytase-like PQQ PQQ_2 PQQ_3 RAG2 RCC1 RCC1_2 Reg_prop SBBP SBP56 SdiA-regulated SGL Str_synth TcdB_toxin_midN TolB_like VCBS WD40 WD40_3 WD40_4 WD40_like


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

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HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_3266 (release 9.0)
Previous IDs: none
Type: Family
Author: Moxon SJ
Number in seed: 33
Number in full: 5431
Average length of the domain: 484.60 aa
Average identity of full alignment: 37 %
Average coverage of the sequence by the domain: 87.36 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 24.2 24.2
Trusted cut-off 24.2 24.3
Noise cut-off 24.1 24.1
Model length: 477
Family (HMM) version: 7
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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There is 1 interaction for this family. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Arylsulfotrans domain has been found. There are 14 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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