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20  structures 525  species 0  interactions 670  sequences 12  architectures

Family: ADC (PF06314)

Summary: Acetoacetate decarboxylase (ADC)

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Acetoacetate decarboxylase Edit Wikipedia article

Acetoacetate decarboxylase
Acetoacetate decarboxylase biounit 3BH3 with inset.png
Acetoacetate decarboxylase dodecamer structure with bound 2-Pentanone bound in its active sites.
EC number
CAS number 9025-03-0
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Acetoacetate decarboxylase (AAD or ADC) is an enzyme involved in both the ketone body production pathway in humans and other mammals, and solventogenesis in bacteria. Acetoacetate decarboxylase plays a key role in solvent production by catalyzing the decarboxylation of acetoacetate, yielding acetone and carbon dioxide.[1] This enzyme has been of particular interest because it is a classic example of how pKa values of ionizable groups in the enzyme active site can be significantly perturbed. Specifically, the pKa value of lysine 115 in the active site is unusually low, allowing for the formation of a Schiff base intermediate and catalysis.[2]

acetoacetic acid Acetoacetate decarboxylase acetone
Acetoacetic acid.png   Acetone-2D-skeletal.svg
Biochem reaction arrow forward NYNN horiz med.png


Acetoacetate decarboxylase is an enzyme with major historical implications, specifically in World War I and in establishing the state of Israel.[3] During the war the Allies needed pure acetone as a solvent for nitro-cellulose, a highly flammable compound that is the main component in gunpowder.[4] In 1916, biochemist and future first president of Israel Chaim Weizmann was the first to isolate Clostridium acetobutylicum, a Gram-positive, anaerobic bacteria in which acetoacetate decarboxylase is found. Weizmann was able to harness the organism’s ability to yield acetone from starch in order to mass-produce explosives during the war.[3] This led the American and British governments to install the process devised by Chaim Weizmann in several large plants in England, France, Canada, and the United States. Through Weizmann’s scientific contributions in World War I, he became close with influential British leaders educating them of his Zionist beliefs.[5] One of them was Arthur Balfour, the man after whom the Balfour Declaration—the first document pronouncing British support in the establishment of a Jewish homeland—was named.

The production of acetone by acetoacetate decarboxylase-containing or clostridial bacteria was utilized in large-scale industrial syntheses in the first half of the twentieth century. In the 1960s, the industry replaced this process with less expensive, more efficient chemical syntheses of acetone from petroleum and petroleum derivatives.[6] However, there has been a growing interest in acetone production that is more environmentally friendly, causing a resurgence in utilizing acetoacetate decarboxylase-containing bacteria.[7] Similarly, isopropanol and butanol fermentation using clostridial species is also becoming popular.


Crystal structure of acetoacetate decarboxylase in complex with an inhibitor, pentan-2-one. Using the residue Lys115, this inhibitor forms an acetyl acetone Schiff base intermediate. Mostly hydrophobic residues, such as Met 97 and Phe27, are found in the active site. This image was generated using PyMOL, from PDB ID 3BH3.[8]

Acetoacetate decarboxylase is a 365 kDa complex with a homododecameric structure.[8] The overall structure consists of antiparallel β-sheets and a central seven-stranded cone-shaped β-barrel. The core of this β-barrel surrounds the active site in each protomer of the enzyme. The active site, consisting of residues such as Phe27, Met97, and Tyr113, is mostly hydrophobic. However, the active site does contain two charged residues: Arg29 and Glu76. Arg29 is thought to play a role in substrate binding, while Glu76 is thought to play a role in the orienting the active site for catalysis. The overall hydrophobic environment of the active site plays a critical role in favoring the neutral amine form of Lys115, a key residue involved in the formation of a Schiff base intermediate. Another important lysine residue, Lys116, is thought to play an important role in the positioning of Lys115 in the active site. Through hydrogen bonds with Ser16 and Met210, Lys116 positions Lys115 in the hydrophobic pocket of the active site to favor the neutral amine form.

Reaction mechanism

general mechanism showing production of acetone and carbon dioxide
Figure 1: General mechanism of acetoacetate decarboxylase, proceeding through a Schiff base intermediate and producing acetone and carbon dioxide [2]

Acetoacetate decarboxylase from Clostridium acetobutylicum catalyzes the decarboxylation of acetoacetate to yield acetone and carbon dioxide (Figure 1). The reaction mechanism proceeds via the formation of a Schiff base intermediate, which is covalently attached to lysine 115 in the active site. The first line of support for this mechanism came from a radiolabeling experiment in which researchers labeled the carbonyl group of acetoacetate with 18O and observed that oxygen exchange to water, used as the solvent, is a necessary part of decarboxylation step.[9] These results provided support that the mechanism proceeds through a Schiff base intermediate between the ketoacid and an amino acid residue on the enzyme.

Further research led to the isolation of an active site peptide sequence and identification of the active site lysine, Lys115, that is involved in the formation of the Schiff base intermediate.[2][10] Additionally, later experiments led to the finding that maximum activity of the enzyme occurs at pH 5.95, suggesting that the pKa of the ε-ammonium group of Lys115 is significantly perturbed in the active site.[2] If the pKa were not perturbed downward, the lysine residue would remain protonated as an ammonium cation, making it unreactive for the nucleophilic addition necessary to form the Schiff base.

Figure 2: Scheme of the reaction, with reporter molecule 5-NSA, used by Westheimer et al. to measure the pKa of Lys115 in the active site.[11]

Building upon this finding, Westheimer et al. directly measured the pKa of Lys115 in the active site using 5-nitrosalicylaldehyde (5-NSA) . Reaction of 5-NSA with acetoacetate decarboxylase and subsequent reduction of the resulting Schiff base with sodium borohydride led to the incorporation of a 2-hydroxy-5-nitrobenzylamino reporter molecule in the active site (Figure 2). Titration of the enzyme with this attached reporter group revealed that the pKa of Lys115 is decreased to 5.9 in the active site.[12] These results were the basis for the proposal that the perturbation in the pKa of Lys115 was due to its proximity to the positively charged ε-ammonium group of Lys116 in the active site.[2] A nearby positive charge could cause unfavorable electrostatic repulsions that weaken the N-H bond of Lys115. Westheimer et al.'s proposal was further supported by site-directed mutagenesis studies. When Lys116 was mutated to cysteine or asparagine, the pKa of Lys115 was found to be significantly elevated to over 9.2, indicating that positively charged Lys116 plays a critical role in determining the pKa of Lys115.[2] Although a crystal structure was not yet solved to provide structural evidence, this proposal was widely accepted and cited as a textbook example of how the active site can be precisely organized to perturb a pKa and affect reactivity.[8]

In 2009, a crystal structure of acetoacetate decarboxylase from Clostridium acetobutylicum was solved, allowing Westheimer et al.'s proposal to be evaluated from a new perspective . From the crystal structure, researchers found that Lys 115 and Lys 116 are oriented in opposite directions and separated by 14.8 Å (Figure 3).[8] This distance makes it unlikely that the positive charge of Lys116 is able to affect the pKa of Lys115. Instead through hydrogen bonds with Ser16 and Met210, Lys116 likely holds Lys115 into position in a hydrophobic pocket of the active site. This positioning disrupts the stability of the protonated ammonium cation of Lys115, suggesting that the perturbation of Lys115's pKa occurs through a 'desolvation effect'.

Figure 3: Orientation of Lysine 115 and 116. In the active site, lysine 115 and 116 point away from each other and are separated by 14.8 Å. This image was generated using PyMOL, from PDB ID 3BH3.[8]

Inactivation and inhibition

Acetoacetate decarboxylase is inhibited by a number of compounds. Acetic anhydride performs a nucleophilic attack on the critical catalytic residue, Lys115, of acetoacetate decarboxylase to inactivate the enzyme.[13] The rate of inactivation was assessed through the hydrolysis of the synthetic substrate 2,4-dinitrophenyl propionate to dinitrophenol by acetoacetate decarboxylase. In the presence of acetic anhydride, the enzyme is inactivated, unable to catalyze the hydrolysis reaction 2,4-dinitrophenyl propionate to dinitrophenol.[14]

Acetonylsulfonate acts as a competitive inhibitor (KI=8.0 mM ) as it mimics the characteristics of the natural substrate, acetoacetate (KM=8.0 mM).[15] The monoanion version of acetonylphosphonate is also a good inhibitor (KI=0.8mM), more efficient than the acetonylphosphonate monoester or dianion.[16] These findings indicate that active site is very discriminatory and sterically restricted.

Hydrogen cyanide seems to be an uncompetitive inhibitor, combining with Schiff’s base compounds formed at the active site.[15] Addition of carbonyl compounds to the enzyme, in the presence of hydrogen cyanide, increases hydrogen cyanide’s ability to inhibit acetoacetate decarboxylase suggesting that carbonyl compounds readily form Schiff’s bases at the active site. Hydrogen cyanide is most potent as an inhibitor at pH 6, the optimum pH for the enzyme, suggesting that the rate-limiting step of catalysis is the formation of the Schiff base intermediate.

Beta-diketones appear to inhibit acetoacetate decarboxylase well but slowly. Acetoacetate decarboxylase has a KM for acetoacetate of 7x10−3 M whereas the enzyme has a KI for benzoylacetone of 1.9x10−6 M.[15] An enamine is most likely formed upon interaction of beta-diketones with free enzyme.

The reaction of acetoacetate decarboxylase with p-chloromercuriphenylsulfonate (CMS) results in decreased catalytic activity upon two equivalents of CMS per enzyme subunit.[15] CMS interacts with two sulfhydryl groups located on each enzyme subunit. Further inactivation occurs upon addition of a third equivalent of CMS per subunit. Addition of free cysteine to the inhibited enzyme is able to reverse CMS inhibition of acetoacetate decarboxylase.

Activity in bacteria

Acetoacetate decarboxylase has been found and studied in the following bacteria in addition to Clostridium acetobutylicum:

Activity in humans and mammals

While this enzyme has not been purified from human tissue, the activity was shown to be present in human blood serum.[17][18]

In humans and other mammals, the conversion of acetoacetate into acetone and carbon dioxide by acetoacetate decarboxylase is a final irreversible step in the ketone-body pathway that supplies the body with a secondary source of energy.[19] In the liver, acetyl co-A formed from fats and lipids are transformed into three ketone bodies: acetone, acetoacetate, and D-β-hydroxybutyrate. Acetoacetate and D-β-hydroxybutyrate are exported to non-hepatic tissues, where they are converted back into acetyl-coA and used for fuel. Acetone and carbon dioxide on the other hand are exhaled, and not allowed to accumulate under normal conditions.

Acetoacetate and D-β-hydroxybutyrate freely interconvert through the action of D-β-hydroxybutyrate dehydrogenase.[19] Subsequently, one function of acetoacetate decarboxylase may be to regulate the concentrations of the other, two 4-carbon ketone bodies.

Clinical significance

Ketone body production increases significantly when the rate of glucose metabolism is insufficient in meeting the body's energy needs. Such conditions include high-fat ketogenic diets, diabetic ketoacidosis, or severe starvation.[20]

Under elevated levels of acetoacetate and D-β-hydroxybutyrate, acetoacetate decarboxylase produces significantly more acetone. Acetone is toxic, and can accumulate in the body under these conditions. Elevated levels of acetone in the human breath can be used to diagnose diabetes.[20]


  1. ^ Peterson DJ, Bennett GN (1990). "Purification of acetoacetate decarboxylase from Clostridium acetobutylicum ATCC cloning of the acetoacetate decarboxylase in Escherichia coli.". Applied and Environmental Microbiology 56 (11): 3491–3498. 
  2. ^ a b c d e f Highbarger, LA; JA Gerlt; GL Kenyon (January 9, 1996). "Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active site lysine 115". Biochemistry 9 (35): 41–46. doi:10.1021/bi9518306. PMID 8555196. 
  3. ^ a b Bormon, S (2009). "New Structure Revisits History". Structural Biology 87 (21): 9. 
  4. ^ "Britannica Online". 
  5. ^ "Jewish Virtual Library". 
  6. ^ "Modeling of ABE Fermentation". 
  7. ^ Collas, Florent; Wouter Kuit; Benjamin Clement; Remy Marchal; Ana M Lopez-Contreras; Frederic Monot (August 21, 2012). "Simultaneous production of isoproponal, butanol, ethanol, and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains". AMB Express 2 (1): 45. doi:10.1186/2191-0855-2-45. PMC 3583297. PMID 22909015. 
  8. ^ a b c d e Ho, Meng-Chiao; Jean-Francois Menetret, Hiro Tsuruta, Karen N. Allen (May 21, 2009). "The origin of the electrostatic pertubation in acetoacetate decarboxylase". Nature 459: 393–397. doi:10.1038/nature07938. 
  9. ^ Hamilton GA, Westheimer FH (1959). "On the Mechanism of Enzymatic Decarboxylation of Acetoacetate". J. Am. Chem. Soc. 81 (23): 6332–6333. doi:10.1021/ja01532a058. 
  10. ^ Warren, Stuart; Burt Zerner, F.H. Westheimer (March 1966). "Acetoacetate Decarboxylase. Identification of Lysine at the Active Site.". Biochemistry 5 (3): 817–823. doi:10.1021/bi00867a002. 
  11. ^ "A History of Acetoacetate Decarboxylase". Retrieved 26 May 2014. 
  12. ^ Kokesh, Fritz C.; F.H. Westheimer (December 29, 1971). "A reporter group at the active site of acetoacetate decarboxylase. Ionization constant of the amino group.". Journal of the American Chemical Society 93 (26): 7270–7274. doi:10.1021/ja00755a025. 
  13. ^ O'Leary, M.H.; F.H. Westheimer (1968). "Acetoacetate decarboxylase. Selective acetylation of enzyme". Biochemistry 7 (3): 913–919. doi:10.1021/bi00843a005. 
  14. ^ Schmidt, Donald E.; F.H. Westheimer (1971). "pK of the Lysine Amino Group at the Active Site of Acetoacetate Decarboxylase". Biochemistry 10 (7): 1249–1253. doi:10.1021/bi00783a023. 
  15. ^ a b c d Autor, Anne P.; I. Fridovich (1970). "The Interactions of Acetoacetate Decarboxylase with Carbonyl Compounds, Hydrogen Cyanide, and an Organic Mercurial". J. Biol. Chem. 245 (20): 5214–5222. 
  16. ^ Kluger, Ronald; Kurt Nakaoka (1974). "Inhibition of Acetoacetate Decarboxylase by Ketophosphonates. Structural and Dyanmic Probes of the Active Site". Biochemistry 13 (5): 910–914. doi:10.1021/bi00702a013. 
  17. ^ van Stekelenburg GJ, Koorevaar G (June 1972). "Evidence for the existence of mammalian acetoacetate decarboxylase: with special reference to human blood serum". Clin. Chim. Acta 39 (1): 191–9. doi:10.1016/0009-8981(72)90316-6. PMID 4624981. 
  18. ^ Koorevaar G, Van Stekelenburg GJ (September 1976). "Mammalian acetoacetate decarboxylase activity. Its distribution in subfractions of human albumin and occurrence in various tissues of the rat". Clin. Chim. Acta 71 (2): 173–83. doi:10.1016/0009-8981(76)90528-3. PMID 963888. 
  19. ^ a b "Human Metabolism". 
  20. ^ a b Galassetti PR, Novak B, Nemet D, Rose-Gottron C, Cooper DM, Meinardi S, Newcomb R, Zaldivar F, Blake DR (2005). "Breath ethanol and acetone as indicators of serum glucose levels: an initial report". Diabetes Technol. Ther. 7 (1): 115–23. doi:10.1089/dia.2005.7.115. PMID 15738709. 

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Acetoacetate decarboxylase (ADC) Provide feedback

This family consists of several acetoacetate decarboxylase (ADC) proteins ( EC:

Literature references

  1. Gerischer U, Durre P; , J Bacteriol 1990;172:6907-6918.: Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. PUBMED:2254264 EPMC:2254264

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR010451

Acetoacetate decarboxylase (ADC) is involved in solventogenesis in certain bacteria, which occurs at the end of the exponential growth phase when there is a metabolic switch from classical sugar fermentation with the production of acetate and butyrate to the re-internalisation and oxidation of these acids to acetate and butanol [PUBMED:11824611]. In Clostridium, SpoOA controls the switch from acid to solvent production. A SpoAO-binding motif occurs in the gene encoding ADC [PUBMED:10972834].

This family also contains the fungal decarboxylase DEC1 encoded by the Tox1B locus, which along with the Tox1A gene product is required for the production of the polyketide T-toxin. The pathogenic fungus Cochliobolus heterostrophus (Drechslera maydis) requires the T-toxin for high virulence to maize with T-cytoplasm [PUBMED:12236595].

Gene Ontology

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Domain organisation

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Pfam Clan

This family is a member of clan ADC-like (CL0403), which has the following description:

Superfamily contains the acetoacetate decarboxylase enzyme family EC:, and a family of uncharacterized proteins from bacteria.

The clan contains the following 2 members:



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Seed source: Pfam-B_12720 (release 9.0)
Previous IDs: none
Type: Family
Author: Moxon SJ
Number in seed: 72
Number in full: 670
Average length of the domain: 225.10 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 74.15 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 29.8 29.8
Trusted cut-off 29.8 29.8
Noise cut-off 29.6 29.6
Model length: 236
Family (HMM) version: 6
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Species distribution

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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ADC domain has been found. There are 20 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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