Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
0  structures 452  species 0  interactions 737  sequences 30  architectures

Family: Glyco_hydro_75 (PF07335)

Summary: Fungal chitosanase of glycosyl hydrolase group 75

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Glycoside hydrolase family 75". More...

Glycoside hydrolase family 75 Edit Wikipedia article

Fungal chitosanase of glycosyl hydrolase group 75
Identifiers
Symbol Glyco_hydro_75
Pfam PF07335
CAZy GH75

In molecular biology, glycoside hydrolase family 75 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]

Glycoside hydrolase family 75 CAZY GH_75 includes enzymes with chitosanase EC 3.2.1.132 activity. This family includes several fungal chitosanase proteins. Chitin, xylan, 6-O-sulphated chitosan and O-carboxymethyl chitin are indigestible by chitosanase.[8]

References

  1. ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. doi:10.1073/pnas.92.15.7090. PMC 41477Freely accessible. PMID 7624375. 
  2. ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779. 
  3. ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 ( Pt 2) (Pt 2): 695–6. PMC 1217404Freely accessible. PMID 8687420. 
  4. ^ "Home". CAZy.org. Retrieved 2018-03-06. 
  5. ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490–5. doi:10.1093/nar/gkt1178. PMID 24270786. 
  6. ^ "Glycoside Hydrolase Family 75". CAZypedia.org. Retrieved 2018-03-06. 
  7. ^ "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology. 28 (1): 3–8. December 2018. doi:10.1093/glycob/cwx089. PMID 29040563. 
  8. ^ Cheng CY, Li YK (December 2000). "An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides". Biotechnol. Appl. Biochem. 32 (3): 197–203. doi:10.1042/ba20000063. PMID 11115392. 

This article incorporates text from the public domain Pfam and InterPro IPR009939

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Fungal chitosanase of glycosyl hydrolase group 75 Provide feedback

This family consists of several fungal chitosanase proteins. Chitin, xylan, 6-O-sulphated chitosan and O-carboxymethyl chitin are indigestible by chitosanase [1]. EC:3.2.1.132. The mechanism is likely to be inverting, and the probable catalytic neutrophile base is Asp, with the probable catalytic proton donor being Glu. (see the Chitosanase web-page from CAZY).

Literature references

  1. Cheng CY, Li YK; , Biotechnol Appl Biochem 2000;32:197-203.: An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides. PUBMED:11115392 EPMC:11115392

  2. Zhang XY, Dai AL, Kuroiwa K, Kodaira R, Nogawa M, Shimosaka M, Okazaki M; , Biosci Biotechnol Biochem 2001;65:977-981.: Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660. PUBMED:11388486 EPMC:11388486


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR009939

This family consists of chitosanase proteins. Chitin, xylan, 6-O-sulphated chitosan and O-carboxymethyl chitin are indigestible by chitosanase [PUBMED:11115392].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(69)
Full
(737)
Representative proteomes UniProt
(1278)
NCBI
(1724)
Meta
(0)
RP15
(81)
RP35
(318)
RP55
(580)
RP75
(887)
Jalview View  View  View  View  View  View  View  View   
HTML View  View               
PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(69)
Full
(737)
Representative proteomes UniProt
(1278)
NCBI
(1724)
Meta
(0)
RP15
(81)
RP35
(318)
RP55
(580)
RP75
(887)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(69)
Full
(737)
Representative proteomes UniProt
(1278)
NCBI
(1724)
Meta
(0)
RP15
(81)
RP35
(318)
RP55
(580)
RP75
(887)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_19431 (release 10.0)
Previous IDs: Chitosanase;
Type: Family
Sequence Ontology: SO:0100021
Author: Moxon SJ
Number in seed: 69
Number in full: 737
Average length of the domain: 155.60 aa
Average identity of full alignment: 35 %
Average coverage of the sequence by the domain: 54.08 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.0 21.0
Trusted cut-off 21.0 21.9
Noise cut-off 19.8 20.9
Model length: 163
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.