Summary: Bacteriophage Mu Gam like protein
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Bacteriophage Mu Gam like protein Provide feedback
This family consists of bacterial and phage Gam proteins. The gam gene of bacteriophage Mu encodes a protein which protects linear double stranded DNA from exonuclease degradation in vitro and in vivo .
Akroyd JE, Clayson E, Higgins NP; , Nucleic Acids Res 1986;14:6901-6914.: Purification of the gam gene-product of bacteriophage Mu and determination of the nucleotide sequence of the gam gene. PUBMED:2945162 EPMC:2945162
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR009951
The Gam protein, originally characterised in Bacteriophage Mu, protects linear double stranded DNA from exonuclease degradation in vitro and in vivo [PUBMED:2945162]. This protein is also found in many bacterial species as part of a suspected prophage. Further studies have shown that Gam is a functional counterpart of the eukaryotic Ku protein, which has key roles in DNA repair and in certain transposition events. Gam displays DNA binding characteristics remarkably similar to those of human Ku [PUBMED:12524520]. In addition, Gam can interfere with Ty1 retrotransposition in Saccharomyces cerevisiae (Baker's yeast). These data reveal structural and functional parallels between bacteriophage Gam and eukaryotic Ku and suggest that their functions have been evolutionarily conserved [PUBMED:12524520].
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|Molecular function||double-stranded DNA binding (GO:0003690)|
|Biological process||DNA protection (GO:0042262)|
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Curation and family details
|Seed source:||Pfam-B_19455 (release 10.0)|
|Number in seed:||27|
|Number in full:||345|
|Average length of the domain:||146.00 aa|
|Average identity of full alignment:||27 %|
|Average coverage of the sequence by the domain:||85.80 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Phage_Mu_Gam domain has been found. There are 2 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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