Summary: Stage II sporulation protein P (SpoIIP)
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This family contains the bacterial stage II sporulation protein P (SpoIIP) (approximately 350 residues long). It has been shown that a block in polar cytokinesis in Bacillus subtilis is mediated partly by transcription of spoIID, spoIIM and spoIIP. This inhibition of polar division is involved in the locking in of asymmetry after the formation of a polar septum during sporulation . Engulfment in Bacillus subtilis is mediated by two complementary systems: the first includes the proteins SpoIID, SpoIIM and SpoIIP (DMP) which carry out the engulfment, and the second includes the SpoIIQ-SpoIIIAGH (Q-AH) zipper, that recruits other proteins to the septum in a second-phase of the engulfment. The course of events follows as the incorporation firstly of SpoIIB into the septum during division to serve directly or indirectly as a landmark for localising SpoIIM and then SpoIIP and SpoIID to the septum. SpoIIP and SpoIID interact together to form part of the DMP complex . SpoIIP itself has been identified as an autolysin with peptidoglycan hydrolase activity .
Pogliano J, Osborne N, Sharp MD, Abanes-De Mello A, Perez A, Sun YL, Pogliano K; , Mol Microbiol. 1999;31:1149-1159.: A vital stain for studying membrane dynamics in bacteria: a novel mechanism controlling septation during Bacillus subtilis sporulation. PUBMED:10096082 EPMC:10096082
Chastanet A, Losick R; , Mol Microbiol. 2007;64:139-152.: Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins. PUBMED:17376078 EPMC:17376078
Aung S, Shum J, Abanes-De Mello A, Broder DH, Fredlund-Gutierrez J, Chiba S, Pogliano K; , Mol Microbiol. 2007;65:1534-1546.: Dual localization pathways for the engulfment proteins during Bacillus subtilis sporulation. PUBMED:17824930 EPMC:17824930
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This tab holds annotation information from the InterPro database.
InterPro entry IPR010897
This family contains the bacterial stage II sporulation protein P (SpoIIP) (approximately 350 residues long). It has been shown that a block in polar cytokinesis in Bacillus subtilis is mediated partly by transcription of spoIID, spoIIM and spoIIP. This inhibition of polar division is involved in the locking in of asymmetry after the formation of a polar septum during sporulation [PUBMED:11886548].
SpoIIP is one of the three genes (spoIID, spoIIM and spoIIP, [PUBMED:8501064, PUBMED:7836306, PUBMED:3011962]), under the control of sigma E, that have been shown to be essential for the engulfment of the forespore by the mother cell. Their products are involved in degradation of the septal peptidoglycan and mutations in spoIID, spoIIM or spoIIP block sporulation at morphological stage II, prior to the stage of engulfment. These three genes are absolutely conserved (sometimes even duplicated) in all endospore formers [PUBMED:12662922].
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This clan contains peptidases belonging to MEROPS clan MH, MC and MF. We also include Nicastrin that is part of the gamma secretase complex and not known to be a peptidase.
The clan contains the following 12 members:Amidase_3 AstE_AspA DUF2172 DUF2817 Nicastrin Peptidase_M14 Peptidase_M17 Peptidase_M18 Peptidase_M20 Peptidase_M28 Peptidase_M42 SpoIIP
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Curation and family details
|Seed source:||Pfam-B_21650 (release 10.0)|
|Author:||Vella Briffa B|
|Number in seed:||49|
|Number in full:||584|
|Average length of the domain:||270.30 aa|
|Average identity of full alignment:||29 %|
|Average coverage of the sequence by the domain:||71.69 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||6|
|Download:||download the raw HMM for this family|
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