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0  structures 72  species 0  interactions 74  sequences 1  architecture

Family: MBA1 (PF07961)

Summary: MBA1-like protein

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

MBA1-like protein Provide feedback

Mba1 is an inner membrane protein that is part of the mitochondrial protein export machinery [1]. It binds to the large subunit of mitochondrial ribosomes and cooperates with the C-terminal ribosome-binding domain of Oxa1, which is a central component of the insertion machinery of the inner membrane. In the absence of both Mba1 and the C-terminus of Oxa1, mitochondrial translation products fail to be properly inserted into the inner membrane and serve as substrates of the matrix chaperone Hsp70 [3]. It is proposed that Mba1 functions as a ribosome receptor that cooperates with Oxa1 in the positioning of the ribosome exit site to the insertion machinery of the inner membrane [3].

Literature references

  1. Rep M, Grivell LA; , FEBS Lett 1996;388:185-188.: MBA1 encodes a mitochondrial membrane-associated protein required for biogenesis of the respiratory chain. PUBMED:8690083 EPMC:8690083

  2. Preuss M, Leonhard K, Hell K, Stuart RA, Neupert W, Herrmann JM; , J Cell Biol 2001;153:1085-1096.: Mba1, a novel component of the mitochondrial protein export machinery of the yeast Saccharomyces cerevisiae. PUBMED:11381092 EPMC:11381092

  3. Ott M, Prestele M, Bauerschmitt H, Funes S, Bonnefoy N, Herrmann JM; , EMBO J. 2006;25:1603-1610.: Mba1, a membrane-associated ribosome receptor in mitochondria. PUBMED:16601683 EPMC:16601683


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR024621

Mba1 is an inner membrane protein that is part of the mitochondrial protein export machinery [PUBMED:8690083, PUBMED:11381092]. It binds to the large subunit of mitochondrial ribosomes and cooperates with the C-terminal ribosome-binding domain of Oxa1, which is a central component of the insertion machinery of the inner membrane. In the absence of both Mba1 and the C terminus of Oxa1, mitochondrial translation products fail to be properly inserted into the inner membrane and serve as substrates of the matrix chaperone Hsp70 [PUBMED:16601683]. It is proposed that Mba1 functions as a ribosome receptor that cooperates with Oxa1 in the positioning of the ribosome exit site to the insertion machinery of the inner membrane [PUBMED:16601683].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan NTF2 (CL0051), which has the following description:

This superfamily contains a variety of enzymes such as Scytalone dehydratase, Delta-5-3-ketosteroid isomerase, Limonene-1,2-epoxide hydrolase among others. The family also includes presumed non-enzymatic homologues such as NTF2.

The clan contains the following 24 members:

CaMKII_AD DUF1348 DUF2358 DUF3225 DUF3804 DUF4440 DUF4467 LEH Lumazine_bd Lumazine_bd_2 MBA1 MecA_N Mtr2 NTF2 PHZA_PHZB Ring_hydroxyl_B Scytalone_dh SnoaL SnoaL_2 SnoaL_3 SnoaL_4 Tim44 VirB8 WI12

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(7)
Full
(74)
Representative proteomes NCBI
(79)
Meta
(0)
RP15
(11)
RP35
(26)
RP55
(43)
RP75
(48)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(7)
Full
(74)
Representative proteomes NCBI
(79)
Meta
(0)
RP15
(11)
RP35
(26)
RP55
(43)
RP75
(48)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(7)
Full
(74)
Representative proteomes NCBI
(79)
Meta
(0)
RP15
(11)
RP35
(26)
RP55
(43)
RP75
(48)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Groocock L
Previous IDs: none
Type: Family
Author: Groocock L, Finn RD
Number in seed: 7
Number in full: 74
Average length of the domain: 223.40 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 77.91 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.4 27.4
Trusted cut-off 27.4 27.4
Noise cut-off 27.2 27.2
Model length: 235
Family (HMM) version: 6
Download: download the raw HMM for this family

Species distribution

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