Summary: Xin repeat
Xin repeat Provide feedback
The repeat has the consensus sequence GDV(K/Q/R)(T/S/G)X(R/K/T) WLFETXPLD. This repeat motif is typically found in the N-terminus of the proteins, with a copy number between 2 and 28 repeats. Direct evidence for binding to and stabilising F-actin has been found  in the human protein Q702N9. The homologues in mouse and chicken localise in the adherens junction complex of the intercalated disc in cardiac muscle and in the myotendon junction of skeletal muscle. mXin may co-localise with Vinculin which is known to attach the actin to the cytoplasmic membrane . It has been shown that the amino-terminus of human xin (CMYA1) binds the EVH1 domain of Mena/VASP/EVL, and the carboxy-terminus binds the, for the filamin family unique, domain 20 of filaminC . This confirms the proposed role of xin repeat containing proteins as F-actin-binding adapter proteins.
Sinn HW, Balsamo J, Lilien J, Lin JJ; , Dev Dyn 2002;225:1-13.: Localization of the novel Xin protein to the adherens junction complex in cardiac and skeletal muscle during development. PUBMED:12203715 EPMC:12203715
Pacholsky D, Vakeel P, Himmel M, Lowe T, Stradal T, Rottner K, Furst DO, van der Ven PF; , J Cell Sci 2004;117:5257-5268.: Xin repeats define a novel actin-binding motif. PUBMED:15454575 EPMC:15454575
Cherepanova O, Orlova A, Galkin VE, van der Ven PF, Furst DO, Jin JP, Egelman EH; , J Mol Biol. 2006;356:714-723.: Xin-repeats and nebulin-like repeats bind to F-actin in a similar manner. PUBMED:16384582 EPMC:16384582
van der Ven PF, Ehler E, Vakeel P, Eulitz S, Schenk JA, Milting H, Micheel B, Furst DO; , Exp Cell Res. 2006;312:2154-2167.: Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP. PUBMED:16631741 EPMC:16631741
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR012510
The repeat has the consensus sequence GDV(K/Q/R)(T/S/G)X(R/K/T) WLFETXPLD. This repeat motif is typically found in the N terminus of the proteins, with a copy number between 2 and 28 repeats. Direct evidence for binding to and stabilising F-actin has been found in the human protein (SWISSPROT) [PUBMED:16631741]. The homologues in mouse and chicken localise in the adherens junction complex of the intercalated disc in cardiac muscle and in the myotendon junction of skeletal muscle. mXin may co-localise with Vinculin which is known to attach the actin to the cytoplasmic membrane [PUBMED:16631741]. It has been shown that the amino-terminus of human xin (CMYA1) binds the EVH1 domain of Mena/VASP/EVL, and the carboxy-terminus binds the, for the filamin family unique, domain 20 of filamin C [PUBMED:12203715]. This confirms the proposed role of xin repeat containing proteins as F-actin-binding adapter proteins.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||cell junction (GO:0030054)|
|Molecular function||actin binding (GO:0003779)|
|Biological process||actin cytoskeleton organization (GO:0030036)|
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Curation and family details
|Seed source:||Wu X|
|Author:||Wu X, Finn RD|
|Number in seed:||60|
|Number in full:||1090|
|Average length of the domain:||16.00 aa|
|Average identity of full alignment:||62 %|
|Average coverage of the sequence by the domain:||7.03 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
|Download:||download the raw HMM for this family|
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