Summary: Mad3/BUB1 homology region 2
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Mad3/BUB1 homology region 2 Provide feedback
This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds BUB3 .
Hardwick KG, Johnston RC, Smith DL, Murray AW; , J Cell Biol 2000;148:871-882.: MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. PUBMED:10704439 EPMC:10704439
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR012572
This domain is required for cell cycle arrest induced by spindle assembly checkpoint (SPC) activation. It is also involved in the nuclear accumulation and kinetochore targeting of proteins Bub1p, Bub3p and Mad3p [PUBMED:15525673].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||nucleus (GO:0005634)|
|Biological process||cell cycle checkpoint (GO:0000075)|
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Curation and family details
|Seed source:||Pfam-B_113144 (release 16.0)|
|Author:||Mistry J, Wood V|
|Number in seed:||8|
|Number in full:||63|
|Average length of the domain:||73.40 aa|
|Average identity of full alignment:||37 %|
|Average coverage of the sequence by the domain:||8.76 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||6|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Mad3_BUB1_II domain has been found. There are 7 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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