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0  structures 1391  species 0  interactions 1841  sequences 40  architectures

Family: PIGA (PF08288)

Summary: PIGA (GPI anchor biosynthesis)

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

PIGA (GPI anchor biosynthesis) Provide feedback

This domain is found on phosphatidylinositol n-acetylglucosaminyltransferase proteins. These proteins are involved in GPI anchor biosynthesis and are associated with disease the paroxysmal nocturnal haemoglobinuria [1].

Literature references

  1. Kulkarni S, Bessler M; , J Leukoc Biol 2002;72:1228-1233.: The effect of GPI-anchor deficiency on apoptosis in mice carrying a Piga gene mutation in hematopoietic cells. PUBMED:12488505 EPMC:12488505


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013234

This domain is found on phosphatidylinositol N-acetylglucosaminyltransferase proteins. These proteins are involved in GPI anchor biosynthesis and are associated with the disease paroxysmal nocturnal haemoglobinuria [ PUBMED:12488505 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(21)
Full
(1841)
Representative proteomes UniProt
(3052)
RP15
(348)
RP35
(825)
RP55
(1405)
RP75
(1870)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(21)
Full
(1841)
Representative proteomes UniProt
(3052)
RP15
(348)
RP35
(825)
RP55
(1405)
RP75
(1870)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(21)
Full
(1841)
Representative proteomes UniProt
(3052)
RP15
(348)
RP35
(825)
RP55
(1405)
RP75
(1870)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_6971 (release 17.0)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Mistry J , Wood V
Number in seed: 21
Number in full: 1841
Average length of the domain: 88.4 aa
Average identity of full alignment: 57 %
Average coverage of the sequence by the domain: 18.49 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.6 27.2
Noise cut-off 26.7 26.9
Model length: 90
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044QJY2 View 3D Structure Click here
A0A077Z5U1 View 3D Structure Click here
A0A0D2GVI7 View 3D Structure Click here
A0A0H5S972 View 3D Structure Click here
A0A0K0DTK4 View 3D Structure Click here
A0A0N4UIH0 View 3D Structure Click here
A0A0R0E7P7 View 3D Structure Click here
A0A1C1CMZ7 View 3D Structure Click here
A0A1D6ENE0 View 3D Structure Click here
A0A1D6ICE7 View 3D Structure Click here
A0A3P7ERJ7 View 3D Structure Click here
A0A3Q0KFF9 View 3D Structure Click here
A0A3Q0KFG7 View 3D Structure Click here
A0A5K4EEL7 View 3D Structure Click here
A0A5S6PJF6 View 3D Structure Click here
A4I939 View 3D Structure Click here
B0S6Z8 View 3D Structure Click here
B7F9D1 View 3D Structure Click here
C0NEB4 View 3D Structure Click here
C1HDG5 View 3D Structure Click here
D4AAY7 View 3D Structure Click here
I1M0D4 View 3D Structure Click here
P32363 View 3D Structure Click here
P37287 View 3D Structure Click here
P87172 View 3D Structure Click here
Q18993 View 3D Structure Click here
Q4CUY7 View 3D Structure Click here
Q54QB2 View 3D Structure Click here
Q585S5 View 3D Structure Click here
Q5A6R7 View 3D Structure Click here
Q64323 View 3D Structure Click here
Q7JUM3 View 3D Structure Click here
Q8IJ83 View 3D Structure Click here
Q94BX4 View 3D Structure Click here
U7PPX2 View 3D Structure Click here

trRosetta Structure

The structural model below was generated by the Baker group with the trRosetta software using the Pfam UniProt multiple sequence alignment.

The InterPro website shows the contact map for the Pfam SEED alignment. Hovering or clicking on a contact position will highlight its connection to other residues in the alignment, as well as on the 3D structure.

Improved protein structure prediction using predicted inter-residue orientations. Jianyi Yang, Ivan Anishchenko, Hahnbeom Park, Zhenling Peng, Sergey Ovchinnikov, David Baker Proceedings of the National Academy of Sciences Jan 2020, 117 (3) 1496-1503; DOI: 10.1073/pnas.1914677117;