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0  structures 110  species 0  interactions 594  sequences 25  architectures

Family: RAP (PF08373)

Summary: RAP domain

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

RAP domain Provide feedback

This domain is found in various eukaryotic species, where it is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain [1]. The domain is involved in plant defence in response to bacterial infection [2,3].

Literature references

  1. Lee I, Hong W; , Trends Biochem Sci 2004;29:567-570.: RAP--a putative RNA-binding domain. PUBMED:15501674 EPMC:15501674

  2. Katiyar-Agarwal S, Gao S, Vivian-Smith A, Jin H;, Genes Dev. 2007;21:3123-3134.: A novel class of bacteria-induced small RNAs in Arabidopsis. PUBMED:18003861 EPMC:18003861

  3. Katiyar-Agarwal S, Jin H;, Annu Rev Phytopathol. 2010;48:225-246.: Role of small RNAs in host-microbe interactions. PUBMED:20687832 EPMC:20687832


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013584

The ~60-residue RAP (an acronym for RNA-binding domain abundant in Apicomplexans) domain is found in various proteins in eukaryotes. It is particularly abundant in apicomplexans and might mediate a range of cellular functions through its potential interactions with RNA [PUBMED:15501674].

The RAP domain consists of multiple blocks of charged and aromatics residues and is predicted to be composed of alpha helical and beta strand structures. Two predicted loop regions that are dominated by glycine and tryptophan residues are found before and after the central beta sheet [PUBMED:15501674].

Some proteins known to contain a RAP domain are listed below:

  • Human hypothetical protein MGC5297,
  • Mammalian FAST kinase domain-containing proteins (FASTKDs),
  • Chlamydomonas reinhardtii chloroplastic trans-splicing factor Raa3.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(79)
Full
(594)
Representative proteomes NCBI
(598)
Meta
(10)
RP15
(160)
RP35
(211)
RP55
(306)
RP75
(425)
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PP/heatmap 1 View  View  View  View  View     
Pfam viewer View  View             

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(79)
Full
(594)
Representative proteomes NCBI
(598)
Meta
(10)
RP15
(160)
RP35
(211)
RP55
(306)
RP75
(425)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(79)
Full
(594)
Representative proteomes NCBI
(598)
Meta
(10)
RP15
(160)
RP35
(211)
RP55
(306)
RP75
(425)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_5583 (release 17.0)
Previous IDs: none
Type: Domain
Author: Fenech M
Number in seed: 79
Number in full: 594
Average length of the domain: 58.80 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 8.24 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.6 22.6
Trusted cut-off 22.6 22.6
Noise cut-off 22.5 22.5
Model length: 58
Family (HMM) version: 5
Download: download the raw HMM for this family

Species distribution

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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