Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
12  structures 817  species 2  interactions 892  sequences 18  architectures

Family: CGI-121 (PF08617)

Summary: Kinase binding protein CGI-121

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Kinase binding protein CGI-121". More...

Kinase binding protein CGI-121 Edit Wikipedia article

CGI-121
PDB 1zd0 EBI.jpg
crystal structure of pfu-542154 conserved hypothetical protein
Identifiers
Symbol CGI-121
Pfam PF08617
InterPro IPR013926
SCOP 1zd0
SUPERFAMILY 1zd0

In molecular biology, the kinase binding protein CGI-121 family of proteins includes the kinase binding protein CGI-121 and its homologues. CGI-121 has been shown to bind to the p53-related protein kinase (PRPK).[1] CGI-121 is part of a conserved protein complex, KEOPS. The KEOPS complex is involved in telomere uncapping and telomere elongation.[2] This family of proteins also include archaeal homologues.

References

  1. ^ Miyoshi A, Kito K, Aramoto T, Abe Y, Kobayashi N, Ueda N (April 2003). "Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein". Biochem. Biophys. Res. Commun. 303 (2): 399–405. doi:10.1016/S0006-291X(03)00333-4. PMID 12659830. 
  2. ^ Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D (March 2006). "A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator". Cell. 124 (6): 1155–68. doi:10.1016/j.cell.2005.12.044. PMID 16564010. 

This article incorporates text from the public domain Pfam and InterPro IPR013926

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Kinase binding protein CGI-121 Provide feedback

CGI-121 has been shown to bind to the p53-related protein kinase (PRPK) [1]. PRPK is a novel protein kinase which binds to and induces phosphorylation of the tumour suppressor protein p53. CGI-121 is part of a conserved protein complex, KEOPS. The KEOPS complex is involved in telomere uncapping and telomere elongation [2]. Interestingly this family also include archaeal homologues, formerly in the DUF509 family. A structure for these proteins has been solved by structural genomics.

Literature references

  1. Miyoshi A, Kito K, Aramoto T, Abe Y, Kobayashi N, Ueda N; , Biochem Biophys Res Commun. 2003;303:399-405.: Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. PUBMED:12659830 EPMC:12659830

  2. Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D; , Cell. 2006;124:1155-1168.: A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. PUBMED:16564010 EPMC:16564010


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR013926

This entry represents the EKC/KEOPS complex subunit Cgi121 from fungi and its homologue, TPRKB from mammals. Interestingly, this family also includes archaeal homologues [PUBMED:18951093].

CGI121 is part of the KEOPS complex, which is involved in is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine [PUBMED:21183954]. The KEOPS complex also plays an important part in telomere uncapping and telomere elongation [PUBMED:16564010]. The complex is required for efficient recruitment of transcriptional coactivators [PUBMED:16564010].

TPRKB has been shown to bind to the p53-related protein kinase (PRPK) [PUBMED:12659830]. PRPK is a novel protein kinase which binds to and induces phosphorylation of the tumour suppressor protein p53.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(167)
Full
(892)
Representative proteomes UniProt
(1433)
NCBI
(1683)
Meta
(42)
RP15
(225)
RP35
(497)
RP55
(754)
RP75
(969)
Jalview View  View  View  View  View  View  View  View  View 
HTML View  View               
PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(167)
Full
(892)
Representative proteomes UniProt
(1433)
NCBI
(1683)
Meta
(42)
RP15
(225)
RP35
(497)
RP55
(754)
RP75
(969)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(167)
Full
(892)
Representative proteomes UniProt
(1433)
NCBI
(1683)
Meta
(42)
RP15
(225)
RP35
(497)
RP55
(754)
RP75
(969)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: manual
Previous IDs: none
Type: Domain
Author: Mistry J, Wood V, Bateman A
Number in seed: 167
Number in full: 892
Average length of the domain: 155.20 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 79.34 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.4 21.4
Trusted cut-off 21.4 21.4
Noise cut-off 21.3 21.2
Model length: 162
Family (HMM) version: 9
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Interactions

There are 2 interactions for this family. More...

CGI-121 Pkinase

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CGI-121 domain has been found. There are 12 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...