Summary: TrwC relaxase
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TrwC relaxase Provide feedback
Relaxases are DNA strand transferases which function during the conjugative cell to cell DNA transfer. TrwC binds to the origin of transfer (oriT) and melts the double helix.
Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Perez-Luque R, Coll M, de la Cruz F; , J Mol Biol. 2006;358:857-869.: Unveiling the Molecular Mechanism of a Conjugative Relaxase: The Structure of TrwC Complexed with a 27-mer DNA Comprising the Recognition Hairpin and the Cleavage Site. PUBMED:16540117 EPMC:16540117
Guasch A, Lucas M, Moncalian G, Cabezas M, Perez-Luque R, Gomis-Ruth FX, de la Cruz F, Coll M; , Nat Struct Biol. 2003;10:1002-1010.: Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC. PUBMED:14625590 EPMC:14625590
Llosa M, Grandoso G, Hernando MA, de la Cruz F; , J Mol Biol. 1996;264:56-67.: Functional domains in protein TrwC of plasmid R388: dissected DNA strand transferase and DNA helicase activities reconstitute protein function. PUBMED:8950267 EPMC:8950267
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Curation and family details
|Number in seed:||34|
|Number in full:||741|
|Average length of the domain:||275.90 aa|
|Average identity of full alignment:||33 %|
|Average coverage of the sequence by the domain:||24.11 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||6|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TrwC domain has been found. There are 20 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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