Summary: Vinculin Binding Site
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Vinculin Binding Site Provide feedback
Vinculin binding sites are predominantly found in talin and talin-like molecules, enabling binding of vinculin to talin, stabilising integrin-mediated cell-matrix junctions. Talin, in turn, links integrins to the actin cytoskeleton. The consensus sequence for Vinculin binding sites is LxxAAxxVAxxVxxLIxxA, with a secondary structure prediction of four amphipathic helices. The hydrophobic residues that define the VBS are themselves 'masked' and are buried in the core of a series of helical bundles that make up the talin rod .
Gingras AR, Vogel KP, Steinhoff HJ, Ziegler WH, Patel B, Emsley J, Critchley DR, Roberts GC, Barsukov IL; , Biochemistry. 2006;45:1805-1817.: Structural and dynamic characterization of a vinculin binding site in the talin rod. PUBMED:16460027 EPMC:16460027
Goult BT, Gingras AR, Bate N, Barsukov IL, Critchley DR, Roberts GC;, FEBS Lett. 2010; [Epub ahead of print]: The domain structure of talin: Residues 1815-1973 form a five-helix bundle containing a cryptic vinculin-binding site. PUBMED:20399778 EPMC:20399778
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR015009
Vinculin binding sites are predominantly found in talin and talin-like molecules, enabling binding of vinculin to talin, stabilising integrin-mediated cell-matrix junctions. Talin, in turn, links integrins to the actin cytoskeleton. The consensus sequence for Vinculin binding sites is LxxAAxxVAxxVxxLIxxA, with a secondary structure prediction of four amphipathic helices. The hydrophobic residues that define the VBS are themselves 'masked' and are buried in the core of a series of helical bundles that make up the talin rod [PUBMED:16460027].
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Curation and family details
|Author:||Mistry J, Sammut SJ|
|Number in seed:||22|
|Number in full:||278|
|Average length of the domain:||116.70 aa|
|Average identity of full alignment:||43 %|
|Average coverage of the sequence by the domain:||7.10 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||5|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the VBS domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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