Summary: Cytokinin dehydrogenase 1, FAD and cytokinin binding
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Cytokinin dehydrogenase 1, FAD and cytokinin binding Provide feedback
Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin .
Malito E, Coda A, Bilyeu KD, Fraaije MW, Mattevi A; , J Mol Biol. 2004;341:1237-1249.: Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis. PUBMED:15321719 EPMC:15321719
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This tab holds annotation information from the InterPro database.
InterPro entry IPR015345
This domain adopts an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. It is predominantly found in plant cytokinin dehydrogenase 1, where it is capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin [PUBMED:15321719].
|Molecular function||cytokinin dehydrogenase activity (GO:0019139)|
|flavin adenine dinucleotide binding (GO:0050660)|
|Biological process||cytokinin metabolic process (GO:0009690)|
|oxidation-reduction process (GO:0055114)|
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This clan consists of a duplicated subdomain in a variety of FAD-liked oxidase/dehydrogenase enzymes.
The clan contains the following 6 members:ALO BBE Chol_subst-bind Cytokin-bind FAD-oxidase_C Lact-deh-memb
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Curation and family details
|Number in seed:||18|
|Number in full:||278|
|Average length of the domain:||242.40 aa|
|Average identity of full alignment:||36 %|
|Average coverage of the sequence by the domain:||52.73 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||5|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Cytokin-bind domain has been found. There are 16 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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