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1  structure 108  species 1  interaction 146  sequences 4  architectures

Family: KcnmB2_inactiv (PF09303)

Summary: KCNMB2, ball and chain domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "KCNMB2". More...

KCNMB2 Edit Wikipedia article

KCNMB2
Protein KCNMB2 PDB 1jo6.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases KCNMB2, potassium calcium-activated channel subfamily M regulatory beta subunit 2
External IDs MGI: 1919663 HomoloGene: 4257 GeneCards: KCNMB2
Gene location (Human)
Chromosome 3 (human)
Chr. Chromosome 3 (human)[1]
Chromosome 3 (human)
Genomic location for KCNMB2
Genomic location for KCNMB2
Band n/a Start 178,272,932 bp[1]
End 178,844,429 bp[1]
RNA expression pattern
PBB GE KCNMB2 221097 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001278911
NM_005832
NM_181361

NM_028231

RefSeq (protein)

NP_001265840
NP_005823
NP_852006

NP_082507

Location (UCSC) Chr 3: 178.27 – 178.84 Mb Chr 3: 31.9 – 32.2 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
KCNMB2, ball and chain domain
PDB 1jo6 EBI.jpg
solution structure of the cytoplasmic n-terminus of the bk beta-subunit kcnmb2
Identifiers
Symbol KcnmB2_inactiv
Pfam PF09303
InterPro IPR015382

Calcium-activated potassium channel subunit beta-2 is a protein that in humans is encoded by the KCNMB2 gene.[5][6]

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can contain two distinct subunits: a pore-forming alpha subunit and a modulatory beta subunit. Each complete MaxiK channel contains four copies of the pore-forming alpha subunit and up to four beta subunits. The protein encoded by the KCNMB2 gene is an auxiliary beta subunit which influences the calcium sensitivity of MaxiK currents and, following activation of MaxiK current, causes persistent inactivation. The subunit encoded by the KCNMB2 gene is expressed in various endocrine cells, including pancreas and adrenal chromaffin cells. It is also found in the brain, including the hippocampus. The KCNMB2 gene is homologous to three other genes found in mammalian genomes: KCNMB1 (found primarily in smooth muscle), KCNMB3, and KCNMB4 (the primary brain MaxiK auxiliary subunit).[6]

Calcium-activated potassium channel subunit beta-2 comprises two domains. An N-terminal cytoplasmic domain, the ball and chain domain, which is responsible for the fast inactivation of these channels,[7] and a C-terminal calcium-activated potassium channel beta subunit domain. The N-terminal domain only occurs in calcium-activated potassium channel subunit beta-2, while the C-terminal domain is found in related proteins.

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000197584 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000037610 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ Wallner M, Meera P, Toro L (May 1999). "Molecular basis of fast inactivation in voltage and Ca2+-activated K+ channels: a transmembrane beta-subunit homolog". Proc Natl Acad Sci U S A. 96 (7): 4137–42. PMC 22433Freely accessible. PMID 10097176. doi:10.1073/pnas.96.7.4137. 
  6. ^ a b "Entrez Gene: KCNMB2 potassium large conductance calcium-activated channel, subfamily M, beta member 2". 
  7. ^ Bentrop D, Beyermann M, Wissmann R, Fakler B (November 2001). "NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels". J. Biol. Chem. 276 (45): 42116–21. PMID 11517232. doi:10.1074/jbc.M107118200. 

Further reading

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This article incorporates text from the public domain Pfam and InterPro IPR015382


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

KCNMB2, ball and chain domain Provide feedback

Members of this family are found in the cytoplasmic N-terminus of KCNMB2, the beta-2 subunit of large conductance calcium and voltage-activated potassium channels. They are responsible for the fast inactivation of these channels [1].

Literature references

  1. Bentrop D, Beyermann M, Wissmann R, Fakler B; , J Biol Chem. 2001;276:42116-42121.: NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels. PUBMED:11517232 EPMC:11517232


This tab holds annotation information from the InterPro database.

InterPro entry IPR015382

This domain is found in the cytoplasmic N terminus of KCNMB2, the beta-2 subunit of large conductance calcium and voltage-activated potassium channels. It is responsible for the fast inactivation of these channels [PUBMED:11517232].

Domain organisation

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Alignments

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(6)
Full
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Representative proteomes UniProt
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NCBI
(436)
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(11)
RP35
(36)
RP55
(99)
RP75
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(6)
Full
(146)
Representative proteomes UniProt
(190)
NCBI
(436)
Meta
(0)
RP15
(11)
RP35
(36)
RP55
(99)
RP75
(131)
Alignment:
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  Seed
(6)
Full
(146)
Representative proteomes UniProt
(190)
NCBI
(436)
Meta
(0)
RP15
(11)
RP35
(36)
RP55
(99)
RP75
(131)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: pdb_1jo6
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Mistry J , Sammut SJ
Number in seed: 6
Number in full: 146
Average length of the domain: 27.00 aa
Average identity of full alignment: 77 %
Average coverage of the sequence by the domain: 11.74 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 26.0 25.1
Noise cut-off 24.9 24.4
Model length: 32
Family (HMM) version: 10
Download: download the raw HMM for this family

Species distribution

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Interactions

There is 1 interaction for this family. More...

CaKB

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the KcnmB2_inactiv domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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