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8  structures 770  species 0  interactions 950  sequences 9  architectures

Family: CENP-O (PF09496)

Summary: Cenp-O kinetochore centromere component

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Cenp-O kinetochore centromere component Provide feedback

This eukaryotic protein is a component of the inner kinetochore subcomplex of the centromere. It has been shown to be involved in chromosome segregation via regulation of the spindle in both yeast and human [1].

Literature references

  1. Liu X, McLeod I, Anderson S, Yates JR 3rd, He X; , EMBO J. 2005;24:2919-2930.: Molecular analysis of kinetochore architecture in fission yeast. PUBMED:16079914 EPMC:16079914

  2. Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW; , Nat Cell Biol. 2006;8:458-469.: The human CENP-A centromeric nucleosome-associated complex. PUBMED:16622419 EPMC:16622419


This tab holds annotation information from the InterPro database.

InterPro entry IPR018464

This entry represents centromere protein O (CENP-O) and its homologues in yeasts, Mcm21 and Mal2.

In humans, centromere protein O (CENP-O) is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation [ PUBMED:16622419 ]. CENP-O mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. CENP-O modulates the kinetochore-bound levels of NDC80 complex [ PUBMED:18007590 ]. It may be involved in incorporation of newly synthesized CENP-A into centromeres via its interaction with the CENPA-NAC complex [ PUBMED:16622420 ].

In Saccharomyces cerevisiae, Mcm21 is a component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p), which links kinetochore subunits with subunits bound to microtubules during kinetochore assembly [ PUBMED:14633972 , PUBMED:22561346 ].

In Schizosaccharomyces pombe, Mal2 is a component of the Sim4 complex, which is required for loading the DASH complex onto the kinetochore via interaction with Dad1 [ PUBMED:12242294 ]. It plays a role in the maintenance of core chromatin structure and kinetochore function [ PUBMED:16079914 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(59)
Full
(950)
Representative proteomes UniProt
(1435)
RP15
(101)
RP35
(349)
RP55
(664)
RP75
(982)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(59)
Full
(950)
Representative proteomes UniProt
(1435)
RP15
(101)
RP35
(349)
RP55
(664)
RP75
(982)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(59)
Full
(950)
Representative proteomes UniProt
(1435)
RP15
(101)
RP35
(349)
RP55
(664)
RP75
(982)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: Wood V
Previous IDs: Cenp-O;
Type: Family
Sequence Ontology: SO:0100021
Author: Wood V , Coggill P , Eberhardt R
Number in seed: 59
Number in full: 950
Average length of the domain: 180.90 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 56.59 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.9 23.9
Trusted cut-off 23.9 23.9
Noise cut-off 23.7 23.8
Model length: 209
Family (HMM) version: 12
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CENP-O domain has been found. There are 8 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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