Summary: MamL-1 domain
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MamL-1 domain Provide feedback
The MamL-1 domain is a polypeptide of up to 70 residues, numbers 15-67 of which adopt an elongated kinked helix that wraps around ANK and CSL forming one of the complexes in the build-up of the Notch transcriptional complex for recruiting general transcription factors.
Nam Y, Sliz P, Song L, Aster JC, Blacklow SC; , Cell. 2006;124:973-983.: Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes. PUBMED:16530044 EPMC:16530044
This tab holds annotation information from the InterPro database.
InterPro entry IPR019082
This entry represents the N-terminal domain found in a family of neurogenic mastermind-like proteins (MAMLs), which act as critical transcriptional co-activators for Notch signaling [PUBMED:18758483, PUBMED:18758478]. Notch receptors are cleaved upon ligand engagement and the intracellular domain of Notch shuttles to the nucleus. MAMLs form a functional DNA-binding complex with the cleaved Notch receptor and the transcription factor CSL, thereby regulating transcriptional events that are specific to the Notch pathway. MAML proteins may also play roles as key transcriptional co-activators in other signal transduction pathways as well, including: muscle differentiation and myopathies (MEF2C) [PUBMED:16510869], tumour suppressor pathway (p53) [PUBMED:17317671] and colon carcinoma survival (beta-catenin) [PUBMED:17875709]. MAML proteins could mediate cross-talk among the various signaling pathways and the diverse activities of the MAML proteins converge to impact normal biological processes and human diseases, including cancers.
The N-terminal domain of MAML proteins adopt an elongated kinked helix that wraps around ANK and CSL forming one of the complexes in the build-up of the Notch transcriptional complex for recruiting general transcription factors. This N-terminal domain is responsible for its interaction with the ankyrin repeat region of the Notch proteins NOTCH1 [PUBMED:16880534], NOTCH2 [PUBMED:17699740], NOTCH3 [PUBMED:19150886] and NOTCH4. It forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1, and also binds CREBBP/CBP [PUBMED:15961999] and CDK8 [PUBMED:15546612]. The C-terminal region is required for transcriptional activation.
|Cellular component||nuclear speck (GO:0016607)|
|Molecular function||transcription coactivator activity (GO:0003713)|
|Biological process||positive regulation of transcription from RNA polymerase II promoter (GO:0045944)|
|Notch signaling pathway (GO:0007219)|
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Curation and family details
|Number in seed:||23|
|Number in full:||108|
|Average length of the domain:||57.20 aa|
|Average identity of full alignment:||45 %|
|Average coverage of the sequence by the domain:||7.44 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 11927849 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||7|
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There are 4 interactions for this family. More...
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MamL-1 domain has been found. There are 4 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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