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4  structures 459  species 0  interactions 474  sequences 10  architectures

Family: ESSS (PF10183)

Summary: ESSS subunit of NADH:ubiquinone oxidoreductase (complex I)

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This is the Wikipedia entry entitled "NADH dehydrogenase (ubiquinone)". More...

NADH dehydrogenase (ubiquinone) Edit Wikipedia article

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This is the Wikipedia entry entitled "NDUFB11". More...

NDUFB11 Edit Wikipedia article

NDUFB11
Identifiers
Aliases NDUFB11, CI-ESSS, ESSS, NP17.3, Np15, P17.3, LSDMCA3, NADH:ubiquinone oxidoreductase subunit B11
External IDs MGI: 1349919 HomoloGene: 10398 GeneCards: NDUFB11
Gene location (Human)
X chromosome (human)
Chr. X chromosome (human)[1]
X chromosome (human)
Genomic location for NDUFB11
Genomic location for NDUFB11
Band Xp11.3 Start 47,142,216 bp[1]
End 47,145,504 bp[1]
RNA expression pattern
PBB GE NDUFB11 218320 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_019056
NM_001135998

NM_019435

RefSeq (protein)

NP_001129470
NP_061929

NP_062308

Location (UCSC) Chr X: 47.14 – 47.15 Mb Chr X: 20.62 – 20.62 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
ESSS subunit of NADH:ubiquinone oxidoreductase (complex I)
Identifiers
Symbol ESSS
Pfam PF10183
InterPro IPR019329

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (NADH-ubiquinone oxidoreductase ESSS subunit) is an enzyme that in humans is encoded by the NDUFB11 gene.[5][6][7] NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 11 is an accessory subunit of the NADH dehydrogenase (ubiquinone) complex, located in the mitochondrial inner membrane. It is also known as Complex I and is the largest of the five complexes of the electron transport chain.[8]

Gene

The NDUFB11 gene is located on the p arm of chromosome X in position 11.23 and is 2,994 base pairs long.[9][10]

Protein

The NDUFB11 protein weighs 17 kDa and is composed of 153 amino acids.[9][10] NDUFB11 is a subunit of the enzyme NADH dehydrogenase (ubiquinone), the largest of the respiratory complexes.

Structure

The structure is L-shaped with a long, hydrophobic transmembrane domain and a hydrophilic domain for the peripheral arm that includes all the known redox centers and the NADH binding site.[8] It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of Complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and that the hydrophobic domain acts as an anchor for the NADH dehydrogenase (ubiquinone) complex at the inner mitochondrial membrane.[7]

Function

The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is not directly involved in catalysis. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.[7] Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.[8]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000147123 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000031059 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ Cui Y, Yu L, Gong R, Zhang M, Fan Y, Yue P, Zhao S (Jun 1999). "Cloning and tissue expressional characterization of a full-length cDNA encoding human neuronal protein P17.3". Biochemical Genetics. 37 (5-6): 175–85. PMID 10544803. doi:10.1023/A:1018734605214. 
  6. ^ Carroll J, Shannon RJ, Fearnley IM, Walker JE, Hirst J (Dec 2002). "Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I. Identification of two new subunits". The Journal of Biological Chemistry. 277 (52): 50311–7. PMID 12381726. doi:10.1074/jbc.M209166200. 
  7. ^ a b c "Entrez Gene: NDUFB11 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa". 
  8. ^ a b c Voet D, Voet JG, Pratt CW (2013). "Chapter 18". Fundamentals of biochemistry: life at the molecular level (4th ed.). Hoboken, NJ: Wiley. pp. 581–620. ISBN 978-0-470-54784-7. 
  9. ^ a b Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P (Oct 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043–53. PMC 4076475Freely accessible. PMID 23965338. doi:10.1161/CIRCRESAHA.113.301151. 
  10. ^ a b "NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). 

Further reading


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) Provide feedback

This subunit is part of the mitochondrial NADH:ubiquinone oxidoreductase (complex I). It carries mitochondrial import sequences [1].

Literature references

  1. Carroll J, Shannon RJ, Fearnley IM, Walker JE, Hirst J; , J Biol Chem. 2002;277:50311-50317.: Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I. Identification of two new subunits. PUBMED:12381726 EPMC:12381726


This tab holds annotation information from the InterPro database.

InterPro entry IPR019329

NADH:ubiquinone oxidoreductase (complex I) (EC) is a respiratory-chain enzyme that catalyses the transfer of two electrons from NADH to ubiquinone in a reaction that is associated with proton translocation across the membrane (NADH + ubiquinone = NAD+ + ubiquinol) [PUBMED:1470679]. Complex I is a major source of reactive oxygen species (ROS) that are predominantly formed by electron transfer from FMNH(2). Complex I is found in bacteria, cyanobacteria (as a NADH-plastoquinone oxidoreductase), archaea [PUBMED:10940377], mitochondria, and in the hydrogenosome, a mitochondria-derived organelle. In general, the bacterial complex consists of 14 different subunits, while the mitochondrial complex contains homologues to these subunits in addition to approximately 31 additional proteins [PUBMED:18394423].

This entry represents the ESSS subunit from mitochondrial NADH:ubiquinone oxidoreductase (complex I). It carries mitochondrial import sequences [PUBMED:12381726].

Domain organisation

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Alignments

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(17)
Full
(474)
Representative proteomes UniProt
(750)
NCBI
(797)
Meta
(0)
RP15
(134)
RP35
(286)
RP55
(391)
RP75
(495)
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  Seed
(17)
Full
(474)
Representative proteomes UniProt
(750)
NCBI
(797)
Meta
(0)
RP15
(134)
RP35
(286)
RP55
(391)
RP75
(495)
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  Seed
(17)
Full
(474)
Representative proteomes UniProt
(750)
NCBI
(797)
Meta
(0)
RP15
(134)
RP35
(286)
RP55
(391)
RP75
(495)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: KOGs (KOG4808)
Previous IDs: none
Type: Family
Author: KOGs, Finn RD, Coggill PC
Number in seed: 17
Number in full: 474
Average length of the domain: 107.40 aa
Average identity of full alignment: 24 %
Average coverage of the sequence by the domain: 74.10 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.8 22.8
Trusted cut-off 22.8 23.1
Noise cut-off 22.3 22.4
Model length: 105
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ESSS domain has been found. There are 4 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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