Summary: Centromere protein Scm3
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Centromere protein Scm3 Provide feedback
Scm3 is a centromere protein that has been shown in Saccharomyces cerevisiae to be required for G2/M progression and Cse4 localisation . The C terminal region of Scm3 proteins is variable in size and sometimes consists of DNA binding motifs .
Stoler S, Rogers K, Weitze S, Morey L, Fitzgerald-Hayes M, Baker RE; , Proc Natl Acad Sci U S A. 2007;104:10571-10576.: Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. PUBMED:17548816 EPMC:17548816
This tab holds annotation information from the InterPro database.
InterPro entry IPR018465
This entry includes human holliday junction recognition protein (HJURP) and its homologue, Scm3 from budding yeasts.
HJURP is a histone chaperone that plays a central role in the incorporation and maintenance of histone H3-like variant CENP-A at centromeres [PUBMED:19410544].
Scm3 is a non-histone component of centromeric chromatin that binds to CenH3-H4 histones, which are required for kinetochore assembly. Scm3 is required for Cse4 (CENP- homologue) localisation and is required for its centromeric association [PUBMED:17574026, PUBMED:17569568]. The histone H3 variant Cse4 replaces conventional histone H3 in centromeric chromatin and helps direct the assembly of the kinetochore. In addition, Scm3 has is required for G2/M progression [PUBMED:17548816] and is required to maintain kinetochore function throughout the cell cycle. Scm3 contains a nuclear export signal (NES). The N-terminal region of Scm3 is well conserved and functions as the CenH3-interacting domain, while the C-terminal region is variable in size and sometimes consists of DNA binding motifs [PUBMED:17704645].
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|Cellular component||nucleus (GO:0005634)|
|Molecular function||histone binding (GO:0042393)|
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Curation and family details
|Seed source:||Pfam-B_19394 (release 21.0)|
|Author:||Mistry J, Wood V|
|Number in seed:||79|
|Number in full:||380|
|Average length of the domain:||53.10 aa|
|Average identity of full alignment:||36 %|
|Average coverage of the sequence by the domain:||7.34 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||8|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Scm3 domain has been found. There are 3 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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