Summary: Anaphase-promoting complex APC subunit CDC26
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Anaphase-promoting complex APC subunit CDC26 Provide feedback
The anaphase-promoting complex (APC) or cyclosome is a cell cycle-regulated ubiquitin-protein ligase that regulates important events in mitosis such as the initiation of anaphase and exit from telophase. The APC, in conjunction with other enzymes, assembles multi-ubiquitin chains on a variety of regulatory proteins thereby targeting them for proteolysis by the 26S proteasome. CDC26 is one of the nine or so subunits identified within APC but its exact function is not known [1]. The APC/C becomes active at the metaphase/anaphase transition and remains active during G1 phase. One mechanism linked to activation of the APC/C is phosphorylation. The yeast APC/C is composed of at least 13 subunits, but the function of many of the subunits is unknown. Hcn1 is the smallest subunit of the S. pombe APC/C, and is found to be essential for cell viability, APC/C integrity, and proper APC/C regulation. In addition, Hcn1 phosphorylation indicates a specific role for the phosphorylation of this subunit late in the cell cycle [2].
Literature references
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Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM; , Proc Natl Acad Sci U S A. 2000;97:8973-8978.: The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. PUBMED:10922056 EPMC:10922056
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Yoon HJ, Feoktistova A, Chen JS, Jennings JL, Link AJ, Gould KL;, J Biol Chem. 2006;281:32284-32293.: Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. PUBMED:16950791 EPMC:16950791
This tab holds annotation information from the InterPro database.
InterPro entry IPR018860
The anaphase-promoting complex (APC) or cyclosome is a multi-subunit E3 protein ubiquitin ligase that regulates important events in mitosis, such as the initiation of anaphase and exit from telophase. The APC, in conjunction with other enzymes, assembles multi-ubiquitin chains on a variety of regulatory proteins, thereby targeting them for proteolysis by the 26S proteasome.
This entry represents subunit CDC26, whose exact function is not known [PUBMED:10922056].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | anaphase-promoting complex (GO:0005680) |
Biological process | anaphase-promoting complex-dependent catabolic process (GO:0031145) |
regulation of mitotic metaphase/anaphase transition (GO:0030071) |
Domain organisation
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Alignments
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Seed (36) |
Full (558) |
Representative proteomes | UniProt (848) |
NCBI (930) |
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RP15 (28) |
RP35 (82) |
RP55 (175) |
RP75 (303) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (36) |
Full (558) |
Representative proteomes | UniProt (848) |
NCBI (930) |
Meta (0) |
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RP15 (28) |
RP35 (82) |
RP55 (175) |
RP75 (303) |
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Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
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Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
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Curation and family details
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Curation
Seed source: | Treefam_TF101057 |
Previous IDs: | APC_CDC26; |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Buljan M |
Number in seed: | 36 |
Number in full: | 558 |
Average length of the domain: | 74.30 aa |
Average identity of full alignment: | 24 % |
Average coverage of the sequence by the domain: | 52.99 % |
HMM information
HMM build commands: |
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 74 | ||||||||||||
Family (HMM) version: | 10 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ANAPC_CDC26 domain has been found. There are 30 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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