Summary: Polyubiquitination (PEST) N-terminal domain of MAGUK
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Polyubiquitination (PEST) N-terminal domain of MAGUK Provide feedback
The residues upstream of this domain are the probable palmitoylation sites, particularly two cysteines. The domain has a putative PEST site at the very start that seems to be responsible for poly-ubiquitination . PEST domains are polypeptide sequences enriched in proline (P), glutamic acid (E), serine (S) and threonine (T) that target proteins for rapid destruction. The whole domain, in conjunction with a C-terminal domain of the longer protein, is necessary for dimerisation of the whole protein .
Xu W, Schluter OM, Steiner P, Czervionke BL, Sabatini B, Malenka RC; , Neuron. 2008;57:248-262.: Molecular dissociation of the role of PSD-95 in regulating synaptic strength and LTD. PUBMED:18215622 EPMC:18215622
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR019590
The residues upstream of this domain are the probable palmitoylation sites, particularly two cysteines. The domain has a putative PEST site at the very start that seems to be responsible for poly-ubiquitination [PUBMED:8755249]. PEST domains are polypeptide sequences enriched in proline (P), glutamic acid (E), serine (S) and threonine (T) that target proteins for rapid destruction. The whole domain, in conjunction with a C-terminal domain of the longer protein, is necessary for dimerisation of the whole protein [PUBMED:18215622].
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This example describes an architecture with one
Gladomain, followed by two consecutive
EGFdomains, and finally a single
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Curation and family details
|Seed source:||Pfam-B_18049 (release 22.0)|
|Number in seed:||14|
|Number in full:||339|
|Average length of the domain:||70.70 aa|
|Average identity of full alignment:||44 %|
|Average coverage of the sequence by the domain:||10.98 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||4|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MAGUK_N_PEST domain has been found. There are 21 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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