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0  structures 134  species 0  interactions 294  sequences 6  architectures

Family: Med24_N (PF11277)

Summary: Mediator complex subunit 24 N-terminal

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This is the Wikipedia entry entitled "MED24". More...

MED24 Edit Wikipedia article

Mediator complex subunit 24
Symbols MED24 ; ARC100; CRSP100; CRSP4; DRIP100; THRAP4; TRAP100
External IDs OMIM607000 MGI1344385 HomoloGene40795 GeneCards: MED24 Gene
RNA expression pattern
PBB GE THRAP4 213043 s at tn.png
More reference expression data
Species Human Mouse
Entrez 9862 23989
Ensembl ENSG00000008838 ENSMUSG00000017210
UniProt O75448 Q99K74
RefSeq (mRNA) NM_001079518 NM_011869
RefSeq (protein) NP_001072986 NP_035999
Location (UCSC) Chr 17:
40.02 – 40.06 Mb
Chr 11:
98.7 – 98.73 Mb
PubMed search [1] [2]

Mediator of RNA polymerase II transcription subunit 24 is an enzyme that in humans is encoded by the MED24 gene.[1]

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.[1]


MED24 has been shown to interact with Estrogen receptor alpha,[2][3] Cyclin-dependent kinase 8,[3][4] Calcitriol receptor[2][4] and BRCA1.[5]


  1. ^ a b "Entrez Gene: THRAP4 thyroid hormone receptor associated protein 4". 
  2. ^ a b Kitagawa, Hirochika; Fujiki Ryoji, Yoshimura Kimihiro, Mezaki Yoshihiro, Uematsu Yoshikatsu, Matsui Daisuke, Ogawa Satoko, Unno Kiyoe, Okubo Mataichi, Tokita Akifumi, Nakagawa Takeya, Ito Takashi, Ishimi Yukio, Nagasawa Hiromichi, Matsumoto Toshio, Yanagisawa Junn, Kato Shigeaki (Jun 2003). "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome". Cell (United States) 113 (7): 905–17. doi:10.1016/S0092-8674(03)00436-7. ISSN 0092-8674. PMID 12837248. 
  3. ^ a b Kang, Yun Kyoung; Guermah Mohamed; Yuan Chao-Xing; Roeder Robert G (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors α and β through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (5): 2642–7. doi:10.1073/pnas.261715899. ISSN 0027-8424. PMC 122401. PMID 11867769. 
  4. ^ a b Ito, M; Yuan C X; Malik S; Gu W; Fondell J D; Yamamura S; Fu Z Y; Zhang X; Qin J; Roeder R G (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell (UNITED STATES) 3 (3): 361–70. doi:10.1016/S1097-2765(00)80463-3. ISSN 1097-2765. PMID 10198638. 
  5. ^ Wada, Osamu; Oishi Hajime; Takada Ichiro; Yanagisawa Junn; Yano Tetsu; Kato Shigeaki (Aug 2004). "BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220". Oncogene (England) 23 (35): 6000–5. doi:10.1038/sj.onc.1207786. ISSN 0950-9232. PMID 15208681. 

Further reading

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This is the Wikipedia entry entitled "Mediator (coactivator)". More...

Mediator (coactivator) Edit Wikipedia article

Mediator is a multiprotein complex that functions as a transcriptional coactivator in all eukaryotes. It was discovered by Roger D. Kornberg, winner of the 2006 Nobel Prize in Chemistry. It is also referred to in scientific literature as the vitamin D receptor interacting protein (DRIP) coactivator complex and the thyroid hormone receptor-associated proteins (TRAP).

The mediator complex is required for the successful transcription of nearly all class II gene promoters in yeast.[1] It works in the same manner in mammals. The human Mediator complex has 31+ subunits and is 1.2 MDa in size. Its large surface area provides great potential for protein–protein interaction, even though its sequences do not contain many predicted functional domains. Mediator has been shown to associate with general transcription factors, as well as RNA polymerase II, and is essential for activator-dependent transcription. It has also been demonstrated that the Mediator complex is involved in activator-independent transcription, implying that it may provide a fundamental control of the formation of the initiation complex.[2] Mediator functions as a coactivator and binds to the C-terminal domain of RNA polymerase II holoenzyme, acting as a bridge between this enzyme and transcription factors.[3][4]

General structure

The Mediator complex is composed of up to at least 31 subunits in all eukaryotes studied: MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED28, MED29, MED30, MED31, CCNC, and CDK8. There are three fungal-specific components, referred to as Med2, Med3 and Med5.[5]

The subunits form at least three structurally distinct submodules. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation.

  • The head module contains: MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22.
  • The middle module contains: MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31. CSE2/MED9 interacts directly with MED4.
  • The tail module contains: MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16.
  • The CDK8 module contains: MED12, MED13, CCNC and CDK8. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Table of Mediator complex subunits present in humans and model organisms[6]

Subunit No. Human gene C. elegans gene D. melanogaster gene S. cerevisiae gene Sch. pombe gene
MED1 MED1 Sop3/mdt-1.1, 1.2 MED1 MED1 med1
Med2 a MED2
Med3 a PGD1
MED4 MED4 MED4 MED4 med4
Med5 a NUT1
MED6 MED6 MDT-6 MED6 MED6 med6
MED7 MED7 MDT-7/let-49 MED7 MED7 med7
MED8 MED8 MDT-8 MED8 MED8 med8
Med10 MED10 MDT-10 NUT2 med10
Med11 MED11 MDT-11 MED11 MED11 med11
MED12 MED12 MDT-12/dpy-22 MED12 SRB8 srb8
Med12L MED12L
MED13 MED13 MDT-13/let-19 MED13 SSN2 srb9
MED14 MED14 MDT-14/rgr-1 MED14 RGR1 med14
MED15 MED15 mdt-15 MED15 GAL11 YN91_SCHPO b
MED17 MED17 MDT-17 MED17 SRB4 med17
Med18 MED18 MDT-18 MED18 SRB5 med18
Med19 MED19 MDT-19 MED19 med19
Med20 MED20 MDT-20 MED20 SRB2 med20
MED21 MED21 MDT-21 MED21 SRB7 med21
MED22 MED22 MDT-22 MED22 SRB6 med22
MED23 MED23 MDT-23/sur-2 MED23
MED27 MED27 MED27 med27
Med29 MED29 MDT-19 MED29 MED29
MED31 MED31 MDT-31 MED31 SOH1 med31
CCNC CCNC cic-1 CycC SSN8 pch1
CDK8 CDK8 cdk-8 Cdk8 SSN3 srb10
  • a Fungal-specific
  • b Protein-name in Sch. pombe


  1. ^ Biddick R, Young ET (2005). "Yeast mediator and its role in transcriptional regulation". C. R. Biol. 328 (9): 773–82. doi:10.1016/j.crvi.2005.03.004. PMID 16168358. 
  2. ^ Taatjes, D.J. (2010) The human Mediator complex: a versatile, genome-wide regulator of transcription. Trends Biochem. Sci. 35 (6): 315–322
  3. ^ Björklund S, Gustafsson CM (2005). "The yeast Mediator complex and its regulation". Trends Biochem. Sci. 30 (5): 240–4. doi:10.1016/j.tibs.2005.03.008. PMID 15896741. 
  4. ^ Reeves, Wendy M.; Hahn, Steve (January 2003). "Activator-Independent Functions of the Yeast Mediator Sin4 Complex in Preinitiation Complex Formation and Transcription Reinitiation". Molecular and Cellular Biology 23 (1): 349–358. doi:10.1128/MCB.23.1.349-358.2003. PMC 140685. PMID 12482986. 
  5. ^ PMID:15175151
  6. ^ Gene-names derived from UniProtKB as of 12 October 2012

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mediator complex subunit 24 N-terminal Provide feedback

This subunit of the Mediator complex appears to be conserved only from insects to humans. It is essential for correct retinal development in fish. Subunit composition of the mediator contributes to the control of differentiation in the vertebrate CNS as there are divergent functions of the mediator subunits Crsp34/Med27, Trap100/Med24, and Crsp150/Med14 [2].

Literature references

  1. Bourbon HM, Aguilera A, Ansari AZ, Asturias FJ, Berk AJ, Bjorklund S, Blackwell TK, Borggrefe T, Carey M, Carlson M, Conaway JW, Conaway RC, Emmons SW, Fondell JD, Freedman LP, Fukasawa T, Gustafsson CM, Han M, He X, Herman PK, Hinnebusch AG, Holmberg S, , Mol Cell. 2004;14:553-557.: A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. PUBMED:15175151 EPMC:15175151

  2. Durr K, Holzschuh J, Filippi A, Ettl AK, Ryu S, Shepherd IT, Driever W; , Genetics. 2006;174:693-705.: Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development. PUBMED:16582438 EPMC:16582438

  3. Lariviere L, Seizl M, van Wageningen S, Rother S, van de Pasch L, Feldmann H, Strasser K, Hahn S, Holstege FC, Cramer P; , Genes Dev. 2008;22:872-877.: Structure-system correlation identifies a gene regulatory Mediator submodule. PUBMED:18381891 EPMC:18381891

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR021429

This subunit of the Mediator complex appears to be conserved only from insects to humans. It is essential for correct retinal development in fish. Subunit composition of the mediator contributes to the control of differentiation in the vertebrate CNS as there are divergent functions of the mediator subunits Crsp34/Med27, Trap100/Med24, and Crsp150/Med14 [PUBMED:16582438].

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Seed source: manual
Previous IDs: none
Type: Domain
Author: Coggill P
Number in seed: 14
Number in full: 294
Average length of the domain: 567.50 aa
Average identity of full alignment: 36 %
Average coverage of the sequence by the domain: 93.89 %

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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.4 25.2
Noise cut-off 21.2 23.2
Model length: 996
Family (HMM) version: 4
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