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0  structures 124  species 0  interactions 160  sequences 6  architectures

Family: Med30 (PF11315)

Summary: Mediator complex subunit 30

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This is the Wikipedia entry entitled "MED30". More...

MED30 Edit Wikipedia article

Mediator complex subunit 30
Identifiers
Symbols MED30 ; MED30S; THRAP6; TRAP25
External IDs OMIM610237 MGI1917040 HomoloGene12329 GeneCards: MED30 Gene
Orthologs
Species Human Mouse
Entrez 90390 69790
Ensembl ENSG00000164758 ENSMUSG00000038622
UniProt Q96HR3 Q9CQI9
RefSeq (mRNA) NM_001282986 NM_027212
RefSeq (protein) NP_001269915 NP_081488
Location (UCSC) Chr 8:
118.53 – 118.55 Mb
Chr 15:
52.71 – 52.73 Mb
PubMed search [1] [2]
Med30
Identifiers
Symbol Med30
Pfam PF11315
InterPro IPR021019

Mediator of RNA polymerase II transcription subunit 30 is an enzyme that in humans is encoded by the MED30 gene.[1] It represents subunit Med30 of the Mediator complex and is metazoan-specific,[2] having no homologues in yeasts.


Interactions

MED30 has been shown to interact with MED22.[3]

References

  1. ^ "Entrez Gene: THRAP6 thyroid hormone receptor associated protein 6". 
  2. ^ Baek HJ, Malik S, Qin J, Roeder RG (April 2002). "Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s". Mol. Cell. Biol. 22 (8): 2842–52. doi:10.1128/MCB.22.8.2842-2852.2002. PMC 133729. PMID 11909976. 
  3. ^ Sato, Shigeo; Tomomori-Sato Chieri, Banks Charles A S, Sorokina Irina, Parmely Tari J, Kong Stephanie E, Jin Jingji, Cai Yong, Lane William S, Brower Christopher S, Conaway Ronald C, Conaway Joan Weliky (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". J. Biol. Chem. (United States) 278 (17): 15123–7. doi:10.1074/jbc.C300054200. ISSN 0021-9258. PMID 12584197. 

Further reading


This article incorporates text from the public domain Pfam and InterPro IPR021019

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This is the Wikipedia entry entitled "Mediator (coactivator)". More...

Mediator (coactivator) Edit Wikipedia article

Mediator is a multiprotein complex that functions as a transcriptional coactivator in all eukaryotes. It was discovered by Roger D. Kornberg, winner of the 2006 Nobel Prize in Chemistry. It is also referred to in scientific literature as the vitamin D receptor interacting protein (DRIP) coactivator complex and the thyroid hormone receptor-associated proteins (TRAP).

The mediator complex is required for the successful transcription of nearly all class II gene promoters in yeast.[2] It works in the same manner in mammals. The human Mediator complex has 31+ subunits and is 1.2 MDa in size. Its large surface area provides great potential for protein–protein interaction, even though its sequences do not contain many predicted functional domains. Mediator has been shown to associate with general transcription factors, as well as RNA polymerase II, and is essential for activator-dependent transcription. It has also been demonstrated that the Mediator complex is involved in activator-independent transcription, implying that it may provide a fundamental control of the formation of the initiation complex.[3] Mediator functions as a coactivator and binds to the C-terminal domain of RNA polymerase II holoenzyme, acting as a bridge between this enzyme and transcription factors.[4][5]

General structure

The Mediator complex is composed of up to at least 31 subunits in all eukaryotes studied: MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED28, MED29, MED30, MED31, CCNC, and CDK8. There are three fungal-specific components, referred to as Med2, Med3 and Med5.[6]

The subunits form at least three structurally distinct submodules. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation.

  • The head module contains: MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22.
  • The middle module contains: MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31. CSE2/MED9 interacts directly with MED4.
  • The tail module contains: MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16.
  • The CDK8 module contains: MED12, MED13, CCNC and CDK8. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.


Table of Mediator complex subunits present in humans and model organisms[7]

Subunit No. Human gene C. elegans gene D. melanogaster gene S. cerevisiae gene Sch. pombe gene
MED1 MED1 Sop3/mdt-1.1, 1.2 MED1 MED1 med1
Med2 a MED2
Med3 a PGD1
MED4 MED4 MED4 MED4 med4
Med5 a NUT1
MED6 MED6 MDT-6 MED6 MED6 med6
MED7 MED7 MDT-7/let-49 MED7 MED7 med7
MED8 MED8 MDT-8 MED8 MED8 med8
Med9 MED9 MED9 CSE2
Med10 MED10 MDT-10 NUT2 med10
Med11 MED11 MDT-11 MED11 MED11 med11
MED12 MED12 MDT-12/dpy-22 MED12 SRB8 srb8
Med12L MED12L
MED13 MED13 MDT-13/let-19 MED13 SSN2 srb9
MED14 MED14 MDT-14/rgr-1 MED14 RGR1 med14
MED15 MED15 mdt-15 MED15 GAL11 YN91_SCHPO b
MED16 MED16 MED16 SIN4
MED17 MED17 MDT-17 MED17 SRB4 med17
Med18 MED18 MDT-18 MED18 SRB5 med18
Med19 MED19 MDT-19 MED19 med19
Med20 MED20 MDT-20 MED20 SRB2 med20
MED21 MED21 MDT-21 MED21 SRB7 med21
MED22 MED22 MDT-22 MED22 SRB6 med22
MED23 MED23 MDT-23/sur-2 MED23
MED24 MED24 MED24
MED25 MED25 MED25
MED26 MED26 MED26
MED27 MED27 MED27 med27
MED28 MED28 MED28
Med29 MED29 MDT-19 MED29 MED29
MED30 MED30 MED30
MED31 MED31 MDT-31 MED31 SOH1 med31
CCNC CCNC cic-1 CycC SSN8 pch1
CDK8 CDK8 cdk-8 Cdk8 SSN3 srb10
  • a Fungal-specific
  • b Protein-name in Sch. pombe

References

  1. ^ Gene-names derived from UniProtKB as of 12 October 2012
  2. ^ Biddick R, Young ET (2005). "Yeast mediator and its role in transcriptional regulation". C. R. Biol. 328 (9): 773–82. doi:10.1016/j.crvi.2005.03.004. PMID 16168358. 
  3. ^ Taatjes, D.J. (2010) The human Mediator complex: a versatile, genome-wide regulator of transcription. Trends Biochem. Sci. 35 (6): 315–322
  4. ^ Björklund S, Gustafsson CM (2005). "The yeast Mediator complex and its regulation". Trends Biochem. Sci. 30 (5): 240–4. doi:10.1016/j.tibs.2005.03.008. PMID 15896741. 
  5. ^ Reeves, Wendy M.; Hahn, Steve (January 2003). "Activator-Independent Functions of the Yeast Mediator Sin4 Complex in Preinitiation Complex Formation and Transcription Reinitiation". Molecular and Cellular Biology 23 (1): 349–358. doi:10.1128/MCB.23.1.349-358.2003. PMC 140685. PMID 12482986. 
  6. ^ PMID:15175151
  7. ^ Gene-names derived from UniProtKB as of 12 October 2012

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mediator complex subunit 30 Provide feedback

Med30 is a metazoan-specific subunit of Mediator, having no homologues in yeasts.

Literature references

  1. Bourbon HM, Aguilera A, Ansari AZ, Asturias FJ, Berk AJ, Bjorklund S, Blackwell TK, Borggrefe T, Carey M, Carlson M, Conaway JW, Conaway RC, Emmons SW, Fondell JD, Freedman LP, Fukasawa T, Gustafsson CM, Han M, He X, Herman PK, Hinnebusch AG, Holmberg S, , Mol Cell. 2004;14:553-557.: A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. PUBMED:15175151 EPMC:15175151

  2. Baek HJ, Malik S, Qin J, Roeder RG; , Mol Cell Biol. 2002;22:2842-2852.: Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. PUBMED:11909976 EPMC:11909976


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR021019

The Mediator complex is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. On recruitment the Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.

The Mediator complex is composed of at least 31 subunits: MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11.

The subunits form at least three structurally distinct submodules. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation.

  • The head module contains: MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22.
  • The middle module contains: MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31. CSE2/MED9 interacts directly with MED4.
  • The tail module contains: MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16.
  • The CDK8 module contains: MED12, MED13, CCNC and CDK8.

Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Med30 is a metazoan-specific subunit of Mediator [PUBMED:11909976], having no homologues in yeasts.

Domain organisation

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Seed source: Pfam-B_28118 (release 23.0)
Previous IDs: none
Type: Domain
Author: Coggill P
Number in seed: 21
Number in full: 160
Average length of the domain: 136.50 aa
Average identity of full alignment: 50 %
Average coverage of the sequence by the domain: 62.07 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.1 28.1
Trusted cut-off 30.4 32.5
Noise cut-off 27.1 27.6
Model length: 148
Family (HMM) version: 4
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