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0  structures 228  species 0  interactions 282  sequences 7  architectures

Family: Med28 (PF11594)

Summary: Mediator complex subunit 28

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This is the Wikipedia entry entitled "MED28". More...

MED28 Edit Wikipedia article

MED28
Identifiers
Aliases MED28, 1500003D12Rik, EG1, magicin, mediator complex subunit 28
External IDs MGI: 1914249 HomoloGene: 11873 GeneCards: MED28
Gene location (Human)
Chromosome 4 (human)
Chr. Chromosome 4 (human)[1]
Chromosome 4 (human)
Genomic location for MED28
Genomic location for MED28
Band 4p15.32 Start 17,614,631 bp[1]
End 17,634,105 bp[1]
RNA expression pattern
PBB GE MED28 218438 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_025205

NM_025895

RefSeq (protein)

NP_079481

NP_080171

Location (UCSC) Chr 4: 17.61 – 17.63 Mb Chr 4: 45.52 – 45.53 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
Med28
Identifiers
Symbol Med28
Pfam PF11594
InterPro IPR021640

Mediator of RNA polymerase II transcription subunit 28 is an enzyme that in humans is encoded by the MED28 gene.[5][6][7] It forms part of the Mediator complex.


Function

Subunit Med28 of the Mediator may function as a scaffolding protein within Mediator by maintaining the stability of a submodule within the head module, and components of this submodule act together in a gene-regulatory programme to suppress smooth muscle cell differentiation. Thus, mammalian Mediator subunit Med28 functions as a repressor of smooth muscle-cell differentiation, which could have implications for disorders associated with abnormalities in smooth muscle cell growth and differentiation, including atherosclerosis, asthma, hypertension, and smooth muscle tumours.[8]

Interactions

MED28 has been shown to interact with Merlin,[6] Grb2[6] and MED26.[9]

See also

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000118579 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000015804 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ Liu C, Zhang L, Shao ZM, Beatty P, Sartippour M, Lane TF, Barsky SH, Livingston E, Nguyen M (Jan 2002). "Identification of a novel endothelial-derived gene EG-1". Biochemical and Biophysical Research Communications. 290 (1): 602–12. PMID 11779215. doi:10.1006/bbrc.2001.6119. 
  6. ^ a b c Wiederhold T, Lee MF, James M, Neujahr R, Smith N, Murthy A, Hartwig J, Gusella JF, Ramesh V (Nov 2004). "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2". Oncogene. 23 (54): 8815–25. PMID 15467741. doi:10.1038/sj.onc.1208110. 
  7. ^ "Entrez Gene: MED28 mediator of RNA polymerase II transcription, subunit 28 homolog (S. cerevisiae)". 
  8. ^ Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. PMID 17848560. doi:10.1074/jbc.M706592200. 
  9. ^ Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. PMID 15175163. doi:10.1016/j.molcel.2004.05.006. 

This article incorporates text from the public domain Pfam and InterPro IPR021640

Further reading

  • Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758–65. PMC 311163Freely accessible. PMID 11591653. doi:10.1101/gr.180101. 
  • Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. PMID 12584197. doi:10.1074/jbc.C300054200. 
  • Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry. 279 (7): 5846–51. PMID 14638676. doi:10.1074/jbc.M312523200. 
  • Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. PMID 15175163. doi:10.1016/j.molcel.2004.05.006. 
  • Lu M, Zhang L, Maul RS, Sartippour MR, Norris A, Whitelegge J, Rao JY, Brooks MN (Jul 2005). "The novel gene EG-1 stimulates cellular proliferation". Cancer Research. 65 (14): 6159–66. PMID 16024617. doi:10.1158/0008-5472.CAN-04-4016. 
  • Lee MF, Beauchamp RL, Beyer KS, Gusella JF, Ramesh V (Sep 2006). "Magicin associates with the Src-family kinases and is phosphorylated upon CD3 stimulation". Biochemical and Biophysical Research Communications. 348 (3): 826–31. PMID 16899217. doi:10.1016/j.bbrc.2006.07.126. 
  • Lu M, Zhang L, Sartippour MR, Norris AJ, Brooks MN (Oct 2006). "EG-1 interacts with c-Src and activates its signaling pathway". International Journal of Oncology. 29 (4): 1013–8. PMID 16964398. doi:10.3892/ijo.29.4.1013. 
  • Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. PMID 17848560. doi:10.1074/jbc.M706592200. 


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This is the Wikipedia entry entitled "Mediator (coactivator)". More...

Mediator (coactivator) Edit Wikipedia article

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Mediator complex subunit 28 Provide feedback

Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species [1-2]. It is arranged into four different sections, a core, a head, a tail and a kinase-activity part, and the number of subunits within each of these is what varies with species. Overall, Mediator regulates the transcriptional activity of RNA polymerase II but it would appear that each of the four different sections has a slightly different function [3]. Subunit Med28 of the Mediator may function as a scaffolding protein within Mediator by maintaining the stability of a submodule within the head module, and components of this submodule act together in a gene-regulatory programme to suppress smooth muscle cell differentiation. Thus, mammalian Mediator subunit Med28 functions as a repressor of smooth muscle-cell differentiation, which could have implications for disorders associated with abnormalities in smooth muscle cell growth and differentiation, including atherosclerosis, asthma, hypertension, and smooth muscle tumours [4].

Literature references

  1. Bourbon HM, Aguilera A, Ansari AZ, Asturias FJ, Berk AJ, Bjorklund S, Blackwell TK, Borggrefe T, Carey M, Carlson M, Conaway JW, Conaway RC, Emmons SW, Fondell JD, Freedman LP, Fukasawa T, Gustafsson CM, Han M, He X, Herman PK, Hinnebusch AG, Holmberg S, , Mol Cell. 2004;14:553-557.: A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. PUBMED:15175151 EPMC:15175151

  2. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC; , Mol Cell. 2004;14:685-691.: A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. PUBMED:15175163 EPMC:15175163

  3. Blackwell TK, Walker AK; , WormBook. 2006;5:1-16.: Transcription mechanisms. PUBMED:18050436 EPMC:18050436

  4. Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Naar AM, Ramesh V; , J Biol Chem. 2007;282:32152-32157.: Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. PUBMED:17848560 EPMC:17848560


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Full
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Representative proteomes UniProt
(409)
NCBI
(499)
Meta
(2)
RP15
(67)
RP35
(126)
RP55
(232)
RP75
(283)
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Seed source: manual
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Coggill P
Number in seed: 6
Number in full: 282
Average length of the domain: 80.20 aa
Average identity of full alignment: 39 %
Average coverage of the sequence by the domain: 36.55 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.5 23.5
Trusted cut-off 23.5 23.7
Noise cut-off 23.3 23.4
Model length: 134
Family (HMM) version: 8
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