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0  structures 37  species 0  interactions 60  sequences 4  architectures

Family: AvrE_T3Es (PF11725)

Summary: AvrE-family Type-III effector proteins (T3Es)

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AvrE-family Type-III effector proteins (T3Es) Provide feedback

This family is secreted by gram-negative Gammaproteobacteria such as Pseudomonas syringae of tomato and the fire blight plant pathogen Erwinia amylovora, amongst others. It is an essential pathogenicity factor of approximately 198 kDa. Its injection into the host-plant is dependent upon the bacterial type III or Hrp secretion system [1]. The family is long and carries a number of predicted functional regions, including in Erwinia stewartii (Q9FCY7) an ERMS or endoplasmic reticulum membrane retention signal at both the C- and the N-termini, a leucine-zipper motif from residues 539-560, and a nuclear localisation signal at 1358-1361. this conserved AvrE-family of effectors is among the few that are required for full virulence of many phytopathogenic pseudomonads, erwinias and pantoeas [2]. A double beta-propeller structure is found towards the N-terminus [3]. Furthermore, AvrE1, an AvrE-family T3E from Pseudomonas syringae pv. tomato strain DC3000, associates with specific PP2A B- subunit proteins from its susceptible host Arabidopsis. In a similar fashion, the WtsE (AvrE-family T3E from the maize pathogen Pantoea stewartii subsp. Stewartii) has been shown to target maize protein phosphatase 2A (PP2A) heterotrimeric enzyme complexes via direct interaction with B- regulatory subunits. Taken together, it has been indicated that that sub-component specific PP2A complexes are targeted by bacterial T3Es, including direct targeting by members of the widely conserved AvrE-family. Additionally, DspE, a type-III effector encoded by the broad-host-range bacterial soft-rot pathogen Pectobacterium carotovorum subsp. carotovorum (Pcc), has been indicated to be required and sufficient for disease-associated cell death in host plants [4].

Literature references

  1. Bogdanove AJ, Bauer DW, Beer SV; , J Bacteriol. 1998;180:2244-2247.: Erwinia amylovora secretes DspE, a pathogenicity factor and functional AvrE homolog, through the Hrp (type III secretion) pathway. PUBMED:9555912 EPMC:9555912

  2. Ham JH, Majerczak DR, Arroyo-Rodriguez AS, Mackey DM, Coplin DL; , Mol Plant Microbe Interact. 2006;19:1092-1102.: WtsE, an AvrE-family effector protein from Pantoea stewartii subsp. stewartii, causes disease-associated cell death in corn and requires a chaperone protein for stability. PUBMED:17022173 EPMC:17022173

  3. Sabrina S, Stephane G, Tristan B, Marie-Noelle B, Marie-Anne B;, FEMS Microbiol Lett. 2013;342:54-61.: Mutational analysis of a predicted double beta-propeller domain of the DspA/E effector of Erwinia amylovora. PUBMED:23421848 EPMC:23421848

  4. Jin L, Ham JH, Hage R, Zhao W, Soto-Hernandez J, Lee SY, Paek SM, Kim MG, Boone C, Coplin DL, Mackey D;, PLoS Pathog. 2016;12:e1005609.: Direct and Indirect Targeting of PP2A by Conserved Bacterial Type-III Effector Proteins. PUBMED:27191168 EPMC:27191168


This tab holds annotation information from the InterPro database.

InterPro entry IPR021085

This family is secreted by Gram-negative Gammaproteobacteria such as Pseudomonas syringae of tomato and Erwinia amylovora (Fire blight bacteria), amongst others. It is an essential pathogenicity factor of approximately 198 kDa. Its injection into the host-plant is dependent upon the bacterial type III or Hrp secretion system [PUBMED:9555912]. The family is long and carries a number of predicted functional regions, including an ERMS or endoplasmic reticulum membrane retention signal at both the C- and the N-termini, a leucine-zipper motif from residues 539-560, and a nuclear localisation signal at 1358-1361. This conserved AvrE-family of effectors is among the few that are required for full virulence of many phytopathogenic pseudomonads, erwinias and pantoeas [PUBMED:17022173].

Domain organisation

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Alignments

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(2)
Full
(60)
Representative proteomes UniProt
(540)
NCBI
(1097)
Meta
(0)
RP15
(2)
RP35
(17)
RP55
(54)
RP75
(173)
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  Seed
(2)
Full
(60)
Representative proteomes UniProt
(540)
NCBI
(1097)
Meta
(0)
RP15
(2)
RP35
(17)
RP55
(54)
RP75
(173)
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  Seed
(2)
Full
(60)
Representative proteomes UniProt
(540)
NCBI
(1097)
Meta
(0)
RP15
(2)
RP35
(17)
RP55
(54)
RP75
(173)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: Pfam-B_735 (release 23.0)
Previous IDs: AvrE;
Type: Family
Sequence Ontology: SO:0100021
Author: Mistry J , Coggill P , Eberhardt R , El-Gebali S
Number in seed: 2
Number in full: 60
Average length of the domain: 1007.30 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 92.36 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.0 19.0
Trusted cut-off 19.2 19.1
Noise cut-off 18.8 18.9
Model length: 1778
Family (HMM) version: 8
Download: download the raw HMM for this family

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