Summary: Rpp20 subunit of nuclear RNase MRP and P
Rpp20 subunit of nuclear RNase MRP and P Provide feedback
The nuclear RNase P of Saccharomyces cerevisiae is made up of at least nine protein subunits; Pop1, Pop3, Pop4, Pop5, Pop6, Pop7, Pop8, Rpr2 and Rpp1. Many of these subunits seem to be present also in the RNase MRP, with the exception of Rpr2 (Rpp21) which is unique to RNase P. Human nuclear RNase P and MRP appear to contain at least 10 protein subunits, Rpp14, Rpp20, Rpp21, Rpp25, Rpp29, Rpp30, Rpp38, Rpp40, hPop1 and hPop5, although there is recent evidence that not all of these subunits are shared between P and MRP. Archaeal RNase P has at least four protein subunits homologous to eukaryotic RNase P/MRP proteins . In the yeast RNase P, Pop6 and Pop7 (the Rpp20 homologue) interact with each other and they are both interaction partners of Pop4 ; in the human MRP Rpp25 and Rpp20 interact with each other  and Rpp25 binds to Rpp29 (Pop4) .
Stolc V, Katz A, Altman S;, Proc Natl Acad Sci U S A. 1998;95:6716-6721.: Rpp2, an essential protein subunit of nuclear RNase P, is required for processing of precursor tRNAs and 35S precursor rRNA in Saccharomyces cerevisiae. PUBMED:9618478 EPMC:9618478
Rosenblad MA, Lopez MD, Piccinelli P, Samuelsson T;, Nucleic Acids Res. 2006;34:5145-5156.: Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes. PUBMED:16998185 EPMC:16998185
Perederina A, Esakova O, Koc H, Schmitt ME, Krasilnikov AS;, RNA. 2007;13:1648-1655.: Specific binding of a Pop6/Pop7 heterodimer to the P3 stem of the yeast RNase MRP and RNase P RNAs. PUBMED:17717080 EPMC:17717080
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We make a range of alignments for each Pfam-A family:
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Curation and family details
|Seed source:||Pfam-B_33537 (release 23.0)|
|Author:||Wood V, Coggill P|
|Number in seed:||44|
|Number in full:||133|
|Average length of the domain:||130.40 aa|
|Average identity of full alignment:||29 %|
|Average coverage of the sequence by the domain:||71.76 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||3|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Rpp20 domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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