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5  structures 974  species 0  interactions 1236  sequences 44  architectures

Family: eIF3g (PF12353)

Summary: Eukaryotic translation initiation factor 3 subunit G

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Eukaryotic translation initiation factor 3 subunit G Provide feedback

This domain family is found in eukaryotes, and is approximately 130 amino acids in length. The family is found in association with PF00076. This family is subunit G of the eukaryotic translation initiation factor 3. Subunit G is required for eIF3 integrity.

Literature references

  1. Masutani M, Sonenberg N, Yokoyama S, Imataka H;, EMBO J. 2007;26:3373-3383.: Reconstitution reveals the functional core of mammalian eIF3. PUBMED:17581632 EPMC:17581632


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR024675

At least eleven different protein factors are involved in initiation of protein synthesis in eukaryotes. Binding of initiator tRNA and mRNA to the 40S subunit requires the presence of the translation initiation factors eIF-2 and eIF-3, with eIF-3 being particularly important for 80S ribosome dissociation and mRNA binding [PUBMED:1742346]. eIF-3 is the most complex translation inititation factor, consisting of about 13 putative subunits and having a molecular weight of between 550 - 700 kDa in mammalian cells. Subunits are designated eIF-3a - eIF-3m; the large number of subunits means that the interactions between the individual subunits that make up the eIF-3 complex are complex and varied.

eIF-3G (also termed eIF-3 subunit 4, eIF-3-delta, eIF3-p42, or eIF3-p44) is the RNA-binding subunit of eIF3. Subunit eIF-3G binds 18 S rRNA and beta-globin mRNA, and therefore appears to be a nonspecific RNA-binding protein. It is one of the cytosolic targets and interacts with mature apoptosis-inducing factor (AIF). The yeast orthologue is known as eIF3-p33; it plays an important role in the initiation phase of protein synthesis in yeast. It binds both mRNA and rRNA fragments due to an RNA recognition motif near its C terminus [PUBMED:17581632, PUBMED:9362495, PUBMED:10085088, PUBMED:20679478, PUBMED:16322461, PUBMED:18599441, PUBMED:17322308, PUBMED:16766523, PUBMED:17094969, PUBMED:15703437, PUBMED:14688252, PUBMED:14519125, PUBMED:9973622, PUBMED:9822659].

This entry represents a domain of approximately 130 amino acids in length found at the N terminus of eukaryotic translation initiation factor 3 subunit G. This domain is commonly found in association with the RNA recognition domain .

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Retroviral_zf (CL0511), which has the following description:

This superfamily has zinc-finger domains from GAG proteins, HIV nucleocapsids, nucleocapsid proteins from mason-pfizer monkey virus, and from other nucleic-acid binding proteins. Sometimes the domains appears in duplicate.

The clan contains the following 8 members:

CLIP1_ZNF eIF3g zf-CCHC zf-CCHC_2 zf-CCHC_3 zf-CCHC_4 zf-CCHC_5 zf-CCHC_6

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(109)
Full
(1236)
Representative proteomes UniProt
(1790)
NCBI
(1804)
Meta
(3)
RP15
(345)
RP35
(708)
RP55
(989)
RP75
(1203)
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PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(109)
Full
(1236)
Representative proteomes UniProt
(1790)
NCBI
(1804)
Meta
(3)
RP15
(345)
RP35
(708)
RP55
(989)
RP75
(1203)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(109)
Full
(1236)
Representative proteomes UniProt
(1790)
NCBI
(1804)
Meta
(3)
RP15
(345)
RP35
(708)
RP55
(989)
RP75
(1203)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Gavin OL
Number in seed: 109
Number in full: 1236
Average length of the domain: 118.70 aa
Average identity of full alignment: 34 %
Average coverage of the sequence by the domain: 37.73 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.7 23.7
Trusted cut-off 23.7 23.7
Noise cut-off 23.6 23.6
Model length: 125
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the eIF3g domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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