Summary: RNAPII transcription regulator C-terminal
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RNAPII transcription regulator C-terminal Provide feedback
MMS19 is required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription . This C-terminal domain, along with the N-terminal, MMS19_N, form part of a silencing complex in fission yeast that contains Dos2, Rik1, Mms19 and Cdc20 (the catalytic subunit of DNA polymerase-epsilon). This complex regulates RNA polymerase II (RNA Pol II) activity in heterochromatin and is required for DNA replication and heterochromatin assembly .
Queimado L, Rao M, Schultz RA, Koonin EV, Aravind L, Nardo T, Stefanini M, Friedberg EC;, Nucleic Acids Res. 2001;29:1884-1891.: Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. PUBMED:11328871 EPMC:11328871
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This tab holds annotation information from the InterPro database.
InterPro entry IPR024687
This entry represents the C-terminal domain of MMS19. This domain shares homology with some HEAT repeat sequences. MMS19 is a key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity [PUBMED:22678362, PUBMED:22678361].
In humans, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription [PUBMED:22678362, PUBMED:22678361]. It is also part of the MMXD (MMS19-MIP18-XPD) complex, which plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD [PUBMED:20797633].
In budding yeasts, the mms19 mutants were originally isolated in a screening for mutants hypersensitive to the alkylating agent methyl methanesulfonate (MMS) [PUBMED:8943333]. Different from human MMS19, Mms19 in budding yeasts (also known as Met18) does not participate directly in NER [PUBMED:23585563].
In fission yeast, Mms19 is part of a silencing complex named Rik1-Dos2 complex, which contains Dos2, Rik1, Mms19 and Cdc20. This complex regulates RNA Pol II activity in heterochromatin, and is required for DNA replication and heterochromatin assembly [PUBMED:21725325].
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Curation and family details
|Author:||Gavin OL, Coggill P|
|Number in seed:||94|
|Number in full:||534|
|Average length of the domain:||387.30 aa|
|Average identity of full alignment:||19 %|
|Average coverage of the sequence by the domain:||40.32 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||4|
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