Summary: Mitotic-spindle organizing gamma-tubulin ring associated
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Mitotic-spindle organizing gamma-tubulin ring associated Provide feedback
The name MOZART is derived from letters of 'mitotic-spindle organizing proteins associated with a ring of gamma-tubulin'. This family operates as part of the gamma-tubulin ring complex, gamma-TuRC, one of the complexes necessary for chromosome segregation. This complex is located at centrosomes and mediates the formation of bipolar spindles in mitosis; it consists of six subunits. However, unlike the other four known subunits, this family does not carry the conserved 'Spc97-Spc98' GCP domain, so the TUBCGP nomenclature cannot be used for it. MOZART1 is required for gamma-TuRC recruitment to centrosomes [1].
Literature references
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Hutchins JR, Toyoda Y, Hegemann B, Poser I, Heriche JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM;, Science. 2010; [Epub ahead of print]: Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins. PUBMED:20360068 EPMC:20360068
This tab holds annotation information from the InterPro database.
InterPro entry IPR022214
MOZART1 or Mzt1 is a component of the gamma-tubulin complex and is required for its recruitment to the microtubule organizing centre in humans and yeast [ PUBMED:20360068 , PUBMED:24006493 , PUBMED:23885124 ]. This function is conserved in plant homologues, known as gamma-tubulin complex protein 3 (GCP3)-interacting proteins (GIPs) [ PUBMED:18178112 , PUBMED:22404201 ]. Studies in plant homologues GIP1 and GIP2 indicate that they play a major role in nuclear envelope shaping in both cycling and differentiated cells [ PUBMED:24570680 ] and that they are essential for centromere architecture [ PUBMED:26124146 , PUBMED:26517054 ].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | gamma-tubulin large complex (GO:0000931) |
Biological process | gamma-tubulin complex localization (GO:0033566) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (56) |
Full (1305) |
Representative proteomes | UniProt (1999) |
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RP15 (231) |
RP35 (579) |
RP55 (971) |
RP75 (1340) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (56) |
Full (1305) |
Representative proteomes | UniProt (1999) |
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RP15 (231) |
RP35 (579) |
RP55 (971) |
RP75 (1340) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Prosite |
Previous IDs: | DUF3743; |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: | Gavin OL |
Number in seed: | 56 |
Number in full: | 1305 |
Average length of the domain: | 46.3 aa |
Average identity of full alignment: | 54 % |
Average coverage of the sequence by the domain: | 37.52 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 47 | ||||||||||||
Family (HMM) version: | 11 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Selections
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the MOZART1 domain has been found. There are 11 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.