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0  structures 405  species 0  interactions 417  sequences 6  architectures

Family: Fungal_TACC (PF12709)

Summary: Fungal Transforming acidic coiled-coil (TACC) proteins

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Fungal Transforming acidic coiled-coil (TACC) proteins Provide feedback

TACC proteins are present in different organisms, ranging from yeasts to mammals. Schizosaccharomyces pombe has one TACC protein, known as Alp7 or Mia1p [1]. Alp7/TACC consists of two domains: the N-terminal half that contains the nuclear localization signal (NLS) and has no homology to the other TACC members, and the C-terminal half, which consists of the conserved coiled-coil TACC domain [2]. Alp7/TACC forms a complex with Alp14/TOG and localizes to microtubules, SPBs and kinetochores [2, 3]. This complex is necessary for mitotic and meiotic spindle assembly and proper chromosome segregation [4]. The homologue from S. cerevisiae, Slk19, also has a role in spindle assembly and stability, and it is required for proper chromosome segregation [5, 6].

Literature references

  1. Peset I, Vernos I;, Trends Cell Biol. 2008;18:379-388.: The TACC proteins: TACC-ling microtubule dynamics and centrosome function. PUBMED:18656360 EPMC:18656360

  2. Sato M, Vardy L, Angel Garcia M, Koonrugsa N, Toda T; , Mol Biol Cell. 2004;15:1609-1622.: Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation. PUBMED:14742702 EPMC:14742702

  3. Garcia MA, Vardy L, Koonrugsa N, Toda T;, EMBO J. 2001;20:3389-3401.: Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint. PUBMED:11432827 EPMC:11432827

  4. Tang NH, Okada N, Fong CS, Arai K, Sato M, Toda T;, FEBS Lett. 2014;588:2814-2821.: Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast. PUBMED:24937146 EPMC:24937146

  5. Pfiz S, Zimmermann J, Hilt W;, Genes Cells. 2002;7:1033-1042.: The yeast kinetochore protein Slk19 is required to prevent aberrant chromosome segregation in meiosis and mitosis. PUBMED:12354097 EPMC:12354097

  6. Zeng X, Kahana JA, Silver PA, Morphew MK, McIntosh JR, Fitch IT, Carbon J, Saunders WS;, J Cell Biol. 1999;146:415-425.: Slk19p is a centromere protein that functions to stabilize mitotic spindles. PUBMED:10427094 EPMC:10427094


This tab holds annotation information from the InterPro database.

InterPro entry IPR024312

TACC proteins are present in different organisms, ranging from yeasts to mammals. Schizosaccharomyces pombe has one TACC protein, known as Alp7 or Mia1p [PUBMED:18656360]. Alp7/TACC consists of two domains: the N-terminal half that contains the nuclear localization signal (NLS) and has no homology to the other TACC members, and the C-terminal half, which consists of the conserved coiled-coil TACC domain [PUBMED:14742702]. Alp7/TACC forms a complex with Alp14/TOG and localizes to microtubules, SPBs and kinetochores [PUBMED:14742702, PUBMED:11432827]. This complex is necessary for mitotic and meiotic spindle assembly and proper chromosome segregation [PUBMED:24937146].

The homologue from S. cerevisiae, Slk19, also has a role in spindle assembly and stability, and it is required for proper chromosome segregation [PUBMED:12354097, PUBMED:10427094].

Domain organisation

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Pfam Clan

This family is a member of clan TACC (CL0679), which has the following description:

TACC proteins are present in different organisms, ranging from yeasts to mammals. For example, TACC proteins can be found in: Caenorhabditis elegans (TAC-1), Drosophila melanogaster (D-TACC), Xenopus laevis (Maskin), and in the fission yeast Schizosaccharomyces pombe (Alp7 also known as Mia1p); by contrast, mammals have three such proteins (TACC1, TACC2 [also known as AZU-1 and ECTACC] and TACC3 [also known as AINT and ERIC1]). Alternative splicing further increases the complexity of the TACC protein family in mammals and flies . The first member of the TACC family to be discovered was identified in a search of genomic regions that are amplified in breast cancer. It was named transforming acidic coiled-coil 1 (TACC1) because of its highly acidic nature, the presence of a predicted coiled-coil domain at its C terminus (now known as the TACC domain), and its ability to promote cellular transformation. one of the extensively characterised TACC proteins, Maskin acts as a factor involved in the regulation of mRNA translation during maturation of Xenopus oocytes. Other TACC family members have also been implicated in various events related to gene regulation, including the regulation of translation, RNA maturation and gene expression. Furthermore, TACC proteins have a role in regulating microtubule assembly such as D-TACC which acts as a Drosophila microtubule-associated and centrosomal protein required for centrosome activity and microtubule assembly during mitosis [1].

The clan contains the following 2 members:

Fungal_TACC TACC_C

Alignments

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  Seed
(19)
Full
(417)
Representative proteomes UniProt
(703)
NCBI
(758)
Meta
(0)
RP15
(32)
RP35
(132)
RP55
(248)
RP75
(413)
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  Seed
(19)
Full
(417)
Representative proteomes UniProt
(703)
NCBI
(758)
Meta
(0)
RP15
(32)
RP35
(132)
RP55
(248)
RP75
(413)
Alignment:
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  Seed
(19)
Full
(417)
Representative proteomes UniProt
(703)
NCBI
(758)
Meta
(0)
RP15
(32)
RP35
(132)
RP55
(248)
RP75
(413)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: manual
Previous IDs: Kinetocho_Slk19;
Type: Coiled-coil
Sequence Ontology: SO:0001080
Author: Coggill P , Sangrador A , El-Gebali S
Number in seed: 19
Number in full: 417
Average length of the domain: 68.70 aa
Average identity of full alignment: 47 %
Average coverage of the sequence by the domain: 9.18 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.9 27.0
Noise cut-off 26.9 24.9
Model length: 77
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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