Summary: twin BRCT domain
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twin BRCT domain Provide feedback
This is a BRCT domain that appears in duplicate in most member sequences. BRCT domains are peptide- and phosphopeptide-binding modules. BRCT domains are present in a number of proteins involved in DNA checkpoint controls and DNA repair [1,2].
Literature references
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Xu X, Lee J, Stern DF;, J Biol Chem. 2004;279:34091-34094.: Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1. PUBMED:15220350 EPMC:15220350
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Fenech M, Carr AM, Murray J, Watts FZ, Lehmann AR;, Nucleic Acids Res. 1991;19:6737-6741.: Cloning and characterization of the rad4 gene of Schizosaccharomyces pombe; a gene showing short regions of sequence similarity to the human XRCC1 gene. PUBMED:1762905 EPMC:1762905
Internal database links
SCOOP: | BRCT BRCT_2 LIG3_BRCT RTT107_BRCT_5 |
Similarity to PfamA using HHSearch: | BRCT BRCT_2 LIG3_BRCT RTT107_BRCT_5 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR001357
The breast cancer susceptibility gene contains at its C terminus two copies of a conserved domain that was named BRCT for BRCA1 C terminus. This domain of about 95 amino acids is found in a large variety of proteins involved in DNA repair, recombination and cell cycle control [ PUBMED:8673121 , PUBMED:9034168 , PUBMED:9000507 ]. The BRCT domain is not limited to the C-terminal of protein sequences and can be found in multiple copies or in a single copy as in RAP1 and TdT. BRCT domains are often found as tandem-repeat pairs [ PUBMED:15501676 ]. Some data [ PUBMED:9799248 ] indicate that the BRCT domain functions as a protein-protein interaction module.
The structure of the first of the two C-terminal BRCT domains of the human DNA repair protein XRCC1 has been determined by X-ray crystallography [ PUBMED:9799248 ].
Structures of the BRCA1 BRCT domains revealed a basis for a widely utilised head-to-tail BRCT-BRCT oligomerization mode [ PUBMED:11573086 ]. This conserved tandem BRCT architecture facilitates formation of the canonical BRCT phospho-peptide interaction cleft at a groove between the BRCT domains. BRCT domains disrupt peptide binding by directly occluding this peptide binding groove, or by disrupting key conserved BRCT core folding determinants [ PUBMED:15133503 ].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan BRCT-like (CL0459), which has the following description:
The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage.
The clan contains the following 9 members:
BRCT BRCT_2 BRCT_3 Dfp1_Him1_M LIG3_BRCT NIBRIN_BRCT_II PTCB-BRCT RTT107_BRCT_5 RTT107_BRCT_6Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (35) |
Full (9607) |
Representative proteomes | UniProt (15337) |
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RP15 (1758) |
RP35 (4105) |
RP55 (7324) |
RP75 (9856) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
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— not available.
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Seed (35) |
Full (9607) |
Representative proteomes | UniProt (15337) |
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RP15 (1758) |
RP35 (4105) |
RP55 (7324) |
RP75 (9856) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
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HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_181 (release 24.0) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Wood V |
Number in seed: | 35 |
Number in full: | 9607 |
Average length of the domain: | 63.4 aa |
Average identity of full alignment: | 26 % |
Average coverage of the sequence by the domain: | 10.21 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 63 | ||||||||||||
Family (HMM) version: | 10 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the PTCB-BRCT domain has been found. There are 32 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.