Summary: Eukaryotic metallothionein
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Eukaryotic metallothionein Provide feedback
This is a family of eukaryotic metallothioneins.
Internal database links
|Similarity to PfamA using HHSearch:||Metallothio|
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR024306
Metallothioneins (MT) are small proteins that bind heavy metals, such as zinc, copper, cadmium, nickel, etc. They have a high content of cysteine residues that bind the metal ions through clusters of thiolate bonds [PUBMED:1779825, PUBMED:2959513]. An empirical classification into three classes has been proposed by Fowler and coworkers [PUBMED:2959504] and Kojima [PUBMED:1779826]. Members of class I are defined to include polypeptides related in the positions of their cysteines to equine MT-1B, and include mammalian MTs as well as from crustaceans and molluscs. Class II groups MTs from a variety of species, including sea urchins, fungi, insects and cyanobacteria. Class III MTs are atypical polypeptides composed of gamma-glutamylcysteinyl units [PUBMED:2959504].
This original classification system has been found to be limited, in the sense that it does not allow clear differentiation of patterns of structural similarities, either between or within classes. Consequently, all class I and class II MTs (the proteinaceous sequences) have now been grouped into families of phylogenetically-related and thus alignable sequences. This system subdivides the MT superfamily into families, subfamilies, subgroups, and isolated isoforms and alleles.
The metallothionein superfamily comprises all polypeptides that resemble equine renal metallothionein in several respects [PUBMED:2959504]: e.g., low molecular weight; high metal content; amino acid composition with high Cys and low aromatic residue content; unique sequence with characteristic distribution of cysteines, and spectroscopic manifestations indicative of metal thiolate clusters. A MT family subsumes MTs that share particular sequence-specific features and are thought to be evolutionarily related. The inclusion of a MT within a family presupposes that its amino acid sequence is alignable with that of all members. Fifteen MT families have been characterised, each family being identified by its number and its taxonomic range: e.g., Family 1: vertebrate MTs [see http://www.bioc.unizh.ch/mtpage/protali.html].
This entry represents a subgroup of eukaryotic metallothioneins which includes copper-induced metallothioneins from the protist Tetrahymena [PUBMED:11693660].
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This superfamily contains related families of metallothioneins, prokaryotes and eukaryotes.
The clan contains the following 5 members:Metallothi_Euk2 Metallothio Metallothio_6 Metallothio_Euk Metallothio_Pro
We make a range of alignments for each Pfam-A family:
- the curated alignment from which the HMM for the family is built
- the alignment generated by searching the sequence database using the HMM
- Representative Proteomes (RPs) at 15%, 35%, 55% and 75% co-membership thresholds
- alignment generated by searching the NCBI sequence database using the family HMM
- alignment generated by searching the metagenomics sequence database using the family HMM
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Curation and family details
|Seed source:||Wood V|
|Author:||Wood V, Coggill P|
|Number in seed:||4|
|Number in full:||26|
|Average length of the domain:||67.00 aa|
|Average identity of full alignment:||50 %|
|Average coverage of the sequence by the domain:||92.47 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||2|
|Download:||download the raw HMM for this family|
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