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0  structures 1021  species 0  interactions 2359  sequences 146  architectures

Family: eIF3_p135 (PF12807)

Summary: Translation initiation factor eIF3 subunit 135

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Translation initiation factor eIF3 subunit 135 Provide feedback

Translation initiation factor eIF3 is a multi-subunit protein complex required for initiation of protein biosynthesis in eukaryotic cells. The complex promotes ribosome dissociation, the binding of the initiator methionyl-tRNA to the 40 S ribosomal subunit, and mRNA recruitment to the ribosome. The protein product from TIF31 genes in yeast is p135 which associates with the eIF3 but does not seem to be necessary for protein translation initiation [1].

Literature references

  1. Vornlocher HP, Hanachi P, Ribeiro S, Hershey JW;, J Biol Chem. 1999;274:16802-16812.: A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes. PUBMED:10358023 EPMC:10358023


This tab holds annotation information from the InterPro database.

InterPro entry IPR033646

Mutations in the mitochondrial CLU proteins have been shown to result in clustered mitochondria [PUBMED:12952081, PUBMED:9207087, PUBMED:9601101]. CLU proteins include Saccharomyces cerevisiae clustered mitochondria protein (Clu1p, alias translation initiation factor 31/TIF31p), Dictyostelium discoideum clustered mitochondria protein homologue (CluA), Caenorhabditis elegans clustered mitochondria protein homologue (CLUH/ Protein KIAA0664), Drosophila clueless (alias clustered mitochondria protein homologue), Arabidopsis clustered mitochondria protein (CLU, alias friendly mitochondria protein/FMT), and human clustered mitochondria protein homologue (CLUH).

Dictyostelium CluA is involved in mitochondrial dynamics and is necessary for both, mitochondrial fission and fusion [PUBMED:22980139]. Drosophila clueless is essential for cytoplasmic localization and function of cellular mitochondria [PUBMED:23342118]. The Drosophila clu gene interacts genetically with parkin (park, the Drosophila ortholog of a human gene responsible for many familial cases of Parkinson's disease) [PUBMED:19638420]. Arabidopsis CLU/FMT is required for correct mitochondrial distribution and morphology [PUBMED:14617080]. The specific role CLU proteins play in mitochondrial processes in not yet known. In an early study, S. cerevisiae Clu1/TIF31p was reported as sometimes being associated with the elF3 translation initiation factor. The authors noted, however, that its tentative assignment as a subunit of elf3 was uncertain, and to date there has been no direct evidence for a role of this protein in translation [PUBMED:10358023].

This entry represents a central domain in CLU proteins.

Domain organisation

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Alignments

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  Seed
(142)
Full
(2359)
Representative proteomes UniProt
(3801)
NCBI
(4901)
Meta
(1)
RP15
(351)
RP35
(1175)
RP55
(1852)
RP75
(2425)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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  Seed
(142)
Full
(2359)
Representative proteomes UniProt
(3801)
NCBI
(4901)
Meta
(1)
RP15
(351)
RP35
(1175)
RP55
(1852)
RP75
(2425)
Alignment:
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  Seed
(142)
Full
(2359)
Representative proteomes UniProt
(3801)
NCBI
(4901)
Meta
(1)
RP15
(351)
RP35
(1175)
RP55
(1852)
RP75
(2425)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Seed source: Pfam-B_2213
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Wood V , Coggill P
Number in seed: 142
Number in full: 2359
Average length of the domain: 158.10 aa
Average identity of full alignment: 30 %
Average coverage of the sequence by the domain: 11.91 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.0 22.0
Trusted cut-off 22.2 22.0
Noise cut-off 21.9 21.9
Model length: 173
Family (HMM) version: 8
Download: download the raw HMM for this family

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