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5  structures 421  species 0  interactions 507  sequences 20  architectures

Family: zf-ANAPC11 (PF12861)

Summary: Anaphase-promoting complex subunit 11 RING-H2 finger

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Anaphase-promoting complex subunit 11 RING-H2 finger Provide feedback

Apc11 is one of the subunits of the anaphase-promoting complex or cyclosome [1]. The APC subunits are cullin family proteins with ubiquitin ligase activity [2]. Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). Apc11 acts as an E3 enzyme and is responsible for recruiting E2s to the APC and for mediating the subsequent transfer of ubiquitin to APC substrates in vivo. In Saccharomyces cerevisiae this RING-H2 finger protein defines the minimal ubiquitin ligase activity of the APC, and the integrity of the RING-H2 finger is essential for budding yeast cell viability [3].

Literature references

  1. Peters JM;, Nat Rev Mol Cell Biol. 2006;7:644-656.: The anaphase promoting complex/cyclosome: a machine designed to destroy. PUBMED:16896351 EPMC:16896351

  2. Ohta T, Michel JJ, Schottelius AJ, Xiong Y;, Mol Cell. 1999;3:535-541.: ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. PUBMED:10230407 EPMC:10230407

  3. Leverson JD, Joazeiro CA, Page AM, Huang H, Hieter P, Hunter T;, Mol Biol Cell. 2000;11:2315-2325.: The APC11 RING-H2 finger mediates E2-dependent ubiquitination. PUBMED:10888670 EPMC:10888670


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR024991

Apc11 is one of the subunits of the anaphase-promoting complex or cyclosome (APC) [PUBMED:16896351]. The APC subunits are cullin family proteins with ubiquitin ligase activity [PUBMED:10230407]. Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). Apc11 acts as an E3 enzyme and is responsible for recruiting E2s to the APC and for mediating the subsequent transfer of ubiquitin to APC substrates in vivo. In Saccharomyces cerevisiae this RING-H2 finger protein defines the minimal ubiquitin ligase activity of the APC, and the integrity of the RING-H2 finger is essential for budding yeast cell viability [PUBMED:10888670].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan RING (CL0229), which has the following description:

This clan includes the Ring zinc finger domains as well as the U-box domain that appears to have lost the zinc coordinating cysteine residues [1].

The clan contains the following 38 members:

Baculo_IE-1 Baculo_RING DUF1644 FANCL_C IBR Prok-RING_1 Prok-RING_2 Prok-RING_4 RINGv Rtf2 U-box UPF1_Zn_bind zf-ANAPC11 zf-C3H2C3 zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3 zf-C3HC4_4 zf-C3HC4_5 zf-MIZ zf-NOSIP zf-Nse zf-P11 zf-rbx1 zf-RING-like zf-RING_10 zf-RING_11 zf-RING_2 zf-RING_4 zf-RING_5 zf-RING_6 zf-RING_7 zf-RING_9 zf-RING_UBOX zf-UBP zf-UDP zinc_ribbon_16 Zn_ribbon_17

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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(5)
Full
(507)
Representative proteomes NCBI
(7098)
Meta
(290)
RP15
(83)
RP35
(169)
RP55
(245)
RP75
(307)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(5)
Full
(507)
Representative proteomes NCBI
(7098)
Meta
(290)
RP15
(83)
RP35
(169)
RP55
(245)
RP75
(307)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(5)
Full
(507)
Representative proteomes NCBI
(7098)
Meta
(290)
RP15
(83)
RP35
(169)
RP55
(245)
RP75
(307)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: manual
Previous IDs: zf-Apc11;
Type: Family
Author: Coggill P
Number in seed: 5
Number in full: 507
Average length of the domain: 80.70 aa
Average identity of full alignment: 47 %
Average coverage of the sequence by the domain: 46.52 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.4 21.4
Trusted cut-off 21.4 21.4
Noise cut-off 21.3 21.3
Model length: 85
Family (HMM) version: 3
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the zf-ANAPC11 domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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