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0  structures 571  species 0  interactions 768  sequences 28  architectures

Family: Ecm29 (PF13001)

Summary: Proteasome stabiliser

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Proteasome stabiliser Provide feedback

The proteasome consists of two subunits, and the capacity of the proteasome to degrade protein depends crucially on the interaction between these two subunits. This interaction is affected by a wide range of factors including metabolites, such as ATP, and proteasome-associated proteins such as Ecm29. Ecm29 stabilises the interaction between the two subunits.

Literature references

  1. Leggett DS, Hanna J, Borodovsky A, Crosas B, Schmidt M, Baker RT, Walz T, Ploegh H, Finley D;, Mol Cell. 2002;10:495-507.: Multiple associated proteins regulate proteasome structure and function. PUBMED:12408819 EPMC:12408819

  2. Gorbea C, Goellner GM, Teter K, Holmes RK, Rechsteiner M;, J Biol Chem. 2004;279:54849-54861.: Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles. PUBMED:15496406 EPMC:15496406

  3. Kleijnen MF, Roelofs J, Park S, Hathaway NA, Glickman M, King RW, Finley D;, Nat Struct Mol Biol. 2007;14:1180-1188.: Stability of the proteasome can be regulated allosterically through engagement of its proteolytic active sites. PUBMED:18026118 EPMC:18026118


This tab holds annotation information from the InterPro database.

InterPro entry IPR024372

The proteasome (or macropain) (EC) [PUBMED:7682410, PUBMED:2643381, PUBMED:1317508, PUBMED:7697118, PUBMED:8882582] is a multicatalytic proteinase complex in eukaryotes and archaea, and in some bacteria, that is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. In eukaryotes the 20S proteasome is composed of 28 distinct subunits which form a highly ordered ring-shaped structure (20S ring) of about 700 kDa. Proteasome subunits can be classified on the basis of sequence similarities into two groups, alpha (A) and beta (B). The proteasome consists of four stacked rings composed of alpha/beta/beta/alpha subunits. There are seven different alpha subunits and seven different beta subunits [PUBMED:9087403]. Three of the seven beta subunits are peptidases, each with a different specificity. Subunit beta1c (MEROPS identifier T01.010) has a preference for cleaving glutaminyl bonds ("peptidyl-glutamyl-like" or "caspase-like"), subunit beta2c (MEROPS identifier T01.011) has a preference for cleaving arginyl and lysyl bonds ("trypsin-like"), and subunit beta5c (MEROPS identifier T01.012) cleaves after hydrophobic amino acids ("chymotrypsin-like") [PUBMED:2535672]. The proteasome subunits are related to N-terminal nucleophile hydrolases, and the catalytic subunits have an N-terminal threonine nucleophile.

Ecm29 is an adapter/scaffolding protein that tethers the proteasome core particle to the regulatory particle, stabilising the interaction between these two components [PUBMED:12408819, PUBMED:15496406, PUBMED:18026118].

Gene Ontology

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(123)
Full
(768)
Representative proteomes UniProt
(1157)
NCBI
(1532)
Meta
(1)
RP15
(184)
RP35
(392)
RP55
(606)
RP75
(758)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

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  Seed
(123)
Full
(768)
Representative proteomes UniProt
(1157)
NCBI
(1532)
Meta
(1)
RP15
(184)
RP35
(392)
RP55
(606)
RP75
(758)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(123)
Full
(768)
Representative proteomes UniProt
(1157)
NCBI
(1532)
Meta
(1)
RP15
(184)
RP35
(392)
RP55
(606)
RP75
(758)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_682 (release 24.0)
Previous IDs: none
Type: Family
Author: Wood V, Coggill P
Number in seed: 123
Number in full: 768
Average length of the domain: 406.20 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 27.71 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.5 20.5
Trusted cut-off 20.5 21.2
Noise cut-off 20.4 20.2
Model length: 496
Family (HMM) version: 6
Download: download the raw HMM for this family

Species distribution

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