Summary: Putative amidotransferase
"DUF" families are annotated with the Domain of unknown function Wikipedia article. This is a general article, with no specific information about individual Pfam DUFs. If you have information about this particular DUF, please let us know using the "Add annotation" button below.
Putative amidotransferase Provide feedback
This domain contains similarities to other amidotransferase families such as PF00117. Some members of the family lack the likely catalytic residues.
Internal database links
|Similarity to PfamA using HHSearch:||DJ-1_PfpI|
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR025628This entry represents a subset of the functionally diverse DJ-1 domain superfamily. The only functionally and structurally characterised protein in this entry is the Pseudomonas fluorescens isocyanide hydratase InhA, which consists of a single DJ-1 domain [PUBMED:20630867]. The function of the other DJ-1 domains in this entry cannot be predicted as the core fold can evolve diverse functions by subtle modulation of the environment of the conserved, reactive cysteine residue.
- the number of sequences which exhibit this architecture
a textual description of the architecture, e.g. Gla, EGF x 2, Trypsin.
This example describes an architecture with one
Gladomain, followed by two consecutive
EGFdomains, and finally a single
- the UniProt description of the protein sequence
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Most members of this clan are glutaminase enzymes. This superfamily is shown to be related in . The clan also contains the DJ-1/PfpI family that includes the peptidase PfpI that has a catalytic Cys-His-Glu triad that differs from the class I GAT Cys-His-Glu triad.
The clan contains the following 13 members:BPL_N DJ-1_PfpI DUF1355 DUF4066 GATase GATase_3 GATase_5 Glyco_hydro_42M HTS Peptidase_C26 Peptidase_S51 SNO ThuA
We make a range of alignments for each Pfam-A family:
- the curated alignment from which the HMM for the family is built
- the alignment generated by searching the sequence database using the HMM
- Representative Proteomes (RPs) at 15%, 35%, 55% and 75% co-membership thresholds
- alignment generated by searching the NCBI sequence database using the family HMM
- alignment generated by searching the metagenomics sequence database using the family HMM
You can see the alignments as HTML or in three different sequence viewers:
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
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Curation and family details
|Number in seed:||51|
|Number in full:||5657|
|Average length of the domain:||160.80 aa|
|Average identity of full alignment:||25 %|
|Average coverage of the sequence by the domain:||54.62 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||1|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the DUF4066 domain has been found. There are 20 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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