Summary: tRNase Z endonuclease
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This is family of tRNase Z enzymes, that are closely related structurally to the Lactamase_B family members. tRNase Z is the endonuclease that is involved in tRNA 3'-end maturation through removal of the 3'-trailer sequences from tRNA precursors. The fission yeast Schizosaccharomyces pombe contains two candidate tRNase Zs encoded by two essential genes. The first, Q10155 is targeted to the nucleus and has an SV40 nuclear localisation signal at its N-terminus, consisting of four consecutive arginine and lysine residues between residues 208 and 211 (KKRK) that is critical for the NLS function. The second, P87168 is targeted to the mitochondria, with an N-terminal mitochondrial targeting signal within the first 38 residues .
Gan X, Yang J, Li J, Yu H, Dai H, Liu J, Huang Y;, Biochem J. 2011; [Epub ahead of print]: The fission yeast Schizosaccharomyces pombe has two distinct tRNase ZLs encoded by two different genes and differentially targeted to the nucleus and mitochondria. PUBMED:21208191 EPMC:21208191
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This superfamily of enzymes including beta-lactamases, thiolesterases, members of the glyoxalase II family that catalyse the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid all bind two ions of zinc. An additional family of competence proteins essential for natural transformation do not appear to bind zinc, and might be a transporter involved in DNA uptake.
The clan contains the following 7 members:DUF2070 Lactamase_B Lactamase_B_2 Lactamase_B_3 Lactamase_B_4 Lactamase_B_5 PDEase_II
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Curation and family details
|Author:||Wood V, Coggill P|
|Number in seed:||41|
|Number in full:||279|
|Average length of the domain:||61.80 aa|
|Average identity of full alignment:||37 %|
|Average coverage of the sequence by the domain:||7.17 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||1|
|Download:||download the raw HMM for this family|
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