Summary: Regulated-SNARE-like domain
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Regulated-SNARE-like domain Provide feedback
Longin is one of the approximately 26 components required for transporting proteins from the ER to the plasma membrane, via the Golgi apparatus. It is necessary for the steps of the transfer from the ER to the Golgi complex . Longins are the only R-SNAREs that are common to all eukaryotes, and they are characterised by a conserved N-terminal domain with a profilin-like fold called a longin domain .
Wen W, Chen L, Wu H, Sun X, Zhang M, Banfield DK; , Mol Biol Cell. 2006;17:4282-4299.: Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein. PUBMED:16855025 EPMC:16855025
Rossi V, Banfield DK, Vacca M, Dietrich LE, Ungermann C, D'Esposito M, Galli T, Filippini F;, Trends Biochem Sci. 2004;29:682-688.: Longins and their longin domains: regulated SNAREs and multifunctional SNARE regulators. PUBMED:15544955 EPMC:15544955
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR010908
VAMPs (and its homologue synaptobrevins) define a group of SNARE proteins that contain a C-terminal coiled-coil/SNARE domain, in combination with variable N-terminal domains that are used to classify VAMPs: those containing longin N-terminal domains (~150 aa) are referred to as longins, while those with shorter N-termini are referred to as brevins [PUBMED:12914952]. Longins are the only type of VAMP protein found in all eukaryotes, suggesting that their longin domain is essential. The longin domain is thought to exert a regulatory function. Longin domains have been shown to share the same structural fold, a profilin-like globular domain consisting of a five-stranded antiparallel beta-sheet that is sandwiched by an alpha-helix on one side, and two alpha-helices on the other (beta(2)-alpha-beta(3)-alpha(2)).
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Curation and family details
|Number in seed:||180|
|Number in full:||1355|
|Average length of the domain:||84.60 aa|
|Average identity of full alignment:||23 %|
|Average coverage of the sequence by the domain:||37.83 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||1|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Longin domain has been found. There are 17 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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