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0  structures 915  species 0  interactions 2190  sequences 48  architectures

Family: Questin_oxidase (PF14027)

Summary: Questin oxidase-like

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Questin oxidase-like Provide feedback

This family consists of oxidoreductases involved in the synthesis of different polyketides and derivatives. Questin oxidase GedK from Aspergillus terreus catalyzes the ring cleavage of the anthraquinone questin to form benzophenone desmethylsulochrin [1]. It is part of the gene cluster that mediates the biosynthesis of geodin, an intermediate in the biosynthesis of other natural products [2]. MdpL from Emericella nidulans is part of the gene cluster that mediates the biosynthesis of monodictyphenone [3].

Literature references

  1. Fujii I, Ebizuka Y, Sankawa U;, J Biochem. 1988;103:878-883.: A novel anthraquinone ring cleavage enzyme from Aspergillus terreus. PUBMED:3182756 EPMC:3182756

  2. Nielsen MT, Nielsen JB, Anyaogu DC, Holm DK, Nielsen KF, Larsen TO, Mortensen UH;, PLoS One. 2013;8:e72871.: Heterologous reconstitution of the intact geodin gene cluster in Aspergillus nidulans through a simple and versatile PCR based approach. PUBMED:24009710 EPMC:24009710

  3. Chiang YM, Szewczyk E, Davidson AD, Entwistle R, Keller NP, Wang CC, Oakley BR;, Appl Environ Microbiol. 2010;76:2067-2074.: Characterization of the Aspergillus nidulans monodictyphenone gene cluster. PUBMED:20139316 EPMC:20139316


This tab holds annotation information from the InterPro database.

InterPro entry IPR025337

This family consists of oxidoreductases involved in the synthesis of different polyketides and derivatives. Questin oxidase GedK from Aspergillus terreus catalyses the ring cleavage of the anthraquinone questin to form benzophenone desmethylsulochrin [ PUBMED:3182756 ]. It is part of the gene cluster that mediates the biosynthesis of geodin, an intermediate in the biosynthesis of other natural products [ PUBMED:24009710 ]. MdpL from Emericella nidulans is part of the gene cluster that mediates the biosynthesis of monodictyphenone [ PUBMED:20139316 ]. TpcI from Neosartorya fumigata is part of the gene cluster that mediates the biosynthesis of trypacidin, a mycotoxin with antiprotozoal activity and that plays a role in the infection process [ PUBMED:26278536 , PUBMED:26242966 ].

This entry also includes ptaJ from Pestalotiopsis fici, AflY from Aspergillus parasiticus, CPUR_05427 from the phytopathogenic Claviceps purpurea, AgnL3 from Paecilomyces divaricatus and NotM from Aspergillus sp.. PtaJ is part of the gene cluster that mediates the biosynthesis of pestheic acid, a diphenyl ether which is a biosynthetic precursor of the unique chloropupukeananes [ PUBMED:24302702 ]. AflY is part of the gene cluster that mediates the biosynthesis of aflatoxins, the most toxic and carcinogenic compounds among the known mycotoxins [ PUBMED:15094053 , PUBMED:15006741 ]. CPUR_05427 is part of the ergochrome gene cluster that is responsible for the typical purple-black color of the sclerotia containing the toxic ergot alkaloids [ PUBMED:28955461 ]. AgnL3 is part of the gene cluster that mediates the biosynthesis of agnestins, dihydroxy-xanthone metabolites [ PUBMED:30746079 ]. NotM is part of the gene cluster that mediates the biosynthesis of notoamide that is a fungal indole alkaloid [ PUBMED:20722388 ].

Gene Ontology

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(203)
Full
(2190)
Representative proteomes UniProt
(4030)
RP15
(293)
RP35
(949)
RP55
(1620)
RP75
(2474)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(203)
Full
(2190)
Representative proteomes UniProt
(4030)
RP15
(293)
RP35
(949)
RP55
(1620)
RP75
(2474)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(203)
Full
(2190)
Representative proteomes UniProt
(4030)
RP15
(293)
RP35
(949)
RP55
(1620)
RP75
(2474)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Jackhmmer:C7QAE2
Previous IDs: DUF4243;
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A , El-Gebali S
Number in seed: 203
Number in full: 2190
Average length of the domain: 304.00 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 68.96 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.1
Noise cut-off 25.9 26.9
Model length: 347
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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trRosetta Structure

The structural model below was generated by the Baker group with the trRosetta software using the Pfam UniProt multiple sequence alignment.

The InterPro website shows the contact map for the Pfam SEED alignment. Hovering or clicking on a contact position will highlight its connection to other residues in the alignment, as well as on the 3D structure.

Improved protein structure prediction using predicted inter-residue orientations. Jianyi Yang, Ivan Anishchenko, Hahnbeom Park, Zhenling Peng, Sergey Ovchinnikov, David Baker Proceedings of the National Academy of Sciences Jan 2020, 117 (3) 1496-1503; DOI: 10.1073/pnas.1914677117;