Summary: Butirosin biosynthesis protein H, N-terminal
Butirosin biosynthesis protein H, N-terminal Provide feedback
BtrH_N is the N-terminus of the acyl carrier protein:aminoglycoside acyltransferase BtrH. Alternatively it can be referred to as butirosin biosynthesis protein H. BtrH transfers the unique (S)-4-amino-2-hydroxybutyrate (AHBA) side chain, which protects the antibiotic butirosin from several common resistance mechanisms. Butirosin, an aminoglycoside antibiotic produced by Bacillus circulans, exhibits improved antibiotic properties over its parent molecule and retains bactericidal activity toward many aminoglycoside-resistant strains. Butirosin is unique in carrying the AHBA side-chain. BtrH transfers the AHBA from the acyl carrier protein BtrI to the parent aminoglycoside ribostamycin as a gamma-glutamylated dipeptide [1,2].
Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K;, J Antibiot (Tokyo). 2005;58:373-379.: Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073. PUBMED:16156513 EPMC:16156513
Jensen MH, Otten H, Christensen U, Borchert TV, Christensen LL, Larsen S, Leggio LL;, J Mol Biol. 2010;404:100-111.: Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus. PUBMED:20851126 EPMC:20851126
Internal database links
|Similarity to PfamA using HHSearch:||DUF1839 Guanylate_cyc_2 DUF3335 Peptidase_C39_2|
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR026935
Members of this family are often found in the gene neighbourhood, or fused to, non-ribosomal peptide synthetases. They are predicted to function as transpeptidases in peptide metabolite biosynthesis [PUBMED:20023723].
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This clan includes peptidases with the papain-like fold.
The clan contains the following 60 members:Acetyltransf_2 Amidase_5 Amidase_6 BtrH_N CHAP DUF1175 DUF1287 DUF1460 DUF2272 DUF3335 DUF553 EDR1 Guanylate_cyc_2 LRAT NLPC_P60 OTU Peptidase_C1 Peptidase_C10 Peptidase_C101 Peptidase_C12 Peptidase_C16 Peptidase_C1_2 Peptidase_C2 Peptidase_C21 Peptidase_C23 Peptidase_C27 Peptidase_C28 Peptidase_C31 Peptidase_C32 Peptidase_C33 Peptidase_C34 Peptidase_C36 Peptidase_C39 Peptidase_C39_2 Peptidase_C42 Peptidase_C47 Peptidase_C48 Peptidase_C5 Peptidase_C54 Peptidase_C58 Peptidase_C6 Peptidase_C65 Peptidase_C7 Peptidase_C70 Peptidase_C71 Peptidase_C78 Peptidase_C8 Peptidase_C9 Peptidase_C92 Peptidase_C93 Peptidase_C97 Peptidase_C98 Phytochelatin Rad4 Transglut_core Transglut_core2 Transglut_core3 UCH UCH_1 Viral_protease
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Curation and family details
|Previous IDs:||BtrH; Transpep_BrtH;|
|Author:||Iyer LM, Abhiman S, Burroughs AM, Aravind L|
|Number in seed:||148|
|Number in full:||1171|
|Average length of the domain:||130.20 aa|
|Average identity of full alignment:||24 %|
|Average coverage of the sequence by the domain:||35.03 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||2|
|Download:||download the raw HMM for this family|
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