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0  structures 940  species 0  interactions 1171  sequences 19  architectures

Family: BtrH_N (PF14399)

Summary: Butirosin biosynthesis protein H, N-terminal

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Butirosin biosynthesis protein H, N-terminal Provide feedback

BtrH_N is the N-terminus of the acyl carrier protein:aminoglycoside acyltransferase BtrH. Alternatively it can be referred to as butirosin biosynthesis protein H. BtrH transfers the unique (S)-4-amino-2-hydroxybutyrate (AHBA) side chain, which protects the antibiotic butirosin from several common resistance mechanisms. Butirosin, an aminoglycoside antibiotic produced by Bacillus circulans, exhibits improved antibiotic properties over its parent molecule and retains bactericidal activity toward many aminoglycoside-resistant strains. Butirosin is unique in carrying the AHBA side-chain. BtrH transfers the AHBA from the acyl carrier protein BtrI to the parent aminoglycoside ribostamycin as a gamma-glutamylated dipeptide [1,2].

Literature references

  1. Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K;, J Antibiot (Tokyo). 2005;58:373-379.: Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073. PUBMED:16156513 EPMC:16156513

  2. Jensen MH, Otten H, Christensen U, Borchert TV, Christensen LL, Larsen S, Leggio LL;, J Mol Biol. 2010;404:100-111.: Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus. PUBMED:20851126 EPMC:20851126


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR026935

Members of this family are often found in the gene neighbourhood, or fused to, non-ribosomal peptide synthetases. They are predicted to function as transpeptidases in peptide metabolite biosynthesis [PUBMED:20023723].

Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(148)
Full
(1171)
Representative proteomes NCBI
(1143)
Meta
(11)
RP15
(41)
RP35
(105)
RP55
(149)
RP75
(194)
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Format an alignment

  Seed
(148)
Full
(1171)
Representative proteomes NCBI
(1143)
Meta
(11)
RP15
(41)
RP35
(105)
RP55
(149)
RP75
(194)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(148)
Full
(1171)
Representative proteomes NCBI
(1143)
Meta
(11)
RP15
(41)
RP35
(105)
RP55
(149)
RP75
(194)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Manual
Previous IDs: BtrH; Transpep_BrtH;
Type: Family
Author: Iyer LM, Abhiman S, Burroughs AM, Aravind L
Number in seed: 148
Number in full: 1171
Average length of the domain: 130.20 aa
Average identity of full alignment: 24 %
Average coverage of the sequence by the domain: 35.03 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 29.7 29.7
Trusted cut-off 29.7 29.7
Noise cut-off 29.6 29.6
Model length: 134
Family (HMM) version: 2
Download: download the raw HMM for this family

Species distribution

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