Summary: Cut8 dimerisation domain
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Cut8 dimerisation domain Provide feedback
In Schizosaccharomyces pombe, Cut8 is a nuclear envelope protein that physically interacts with and tethers 26S proteasome in the nucleus resulting in the nuclear accumulation of proteasome . Cut8 comprises three functional domains. An N-terminal lysine-rich segment (PF14482) which binds to the proteasome when ubiquitinated, a central dimerisation domain (this entry) and a C-terminal six-helix bundle (PF08559), which shows structural similarity to 14-3-3 phosphoprotein-binding domains. The six-helix bundle is necessary for liposome and cholesterol binding . Cut8 is a proteasome substrate and the N-terminal segment is polyubiquitinated and functions as a degron tag. Ubiquitination of the amino N-terminal segment is essential to the function of Cut8 . Lysine residues in the N-terminal segment of Cut8 are required for physical interaction with proteasome . In fission yeast the function of Cut8 has been demonstrated to be regulated by ubiquitin-conjugating Rhp6/Ubc2/Rad6 and ligating enzymes Ubr1 . Cut8 homologues have been identified in Drosophila melanogaster, Anopheles gambiae and Dictyostelium discoideum .
Takeda K, Tonthat NK, Glover T, Xu W, Koonin EV, Yanagida M, Schumacher MA;, Proc Natl Acad Sci U S A. 2011;108:16950-16955.: Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. PUBMED:21976488 EPMC:21976488
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Curation and family details
This family is new in this Pfam release.
|Seed source:||Wood V|
|Number in seed:||19|
|Number in full:||126|
|Average length of the domain:||37.70 aa|
|Average identity of full alignment:||39 %|
|Average coverage of the sequence by the domain:||11.94 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||1|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Cut8_M domain has been found. There are 3 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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