Summary: Tautomerase enzyme
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This is the Wikipedia entry entitled "4-Oxalocrotonate tautomerase". More...
4-Oxalocrotonate tautomerase Edit Wikipedia article
4-oxalocrotonate tautomerase | |||||||||||
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Identifiers | |||||||||||
Symbol | Taut | ||||||||||
Pfam | PF01361 | ||||||||||
InterPro | IPR004370 | ||||||||||
CDD | cd00491 | ||||||||||
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4-Oxalocrotonate tautomerase (EC 5.3.2.-4-OT) is an enzyme that converts 2-hydroxymuconate to the αβ-unsaturated ketone, 2-oxo-3-hexenedioate.[1] This enzyme forms part of a bacterial metabolic pathway that oxidatively catabolizes toluene, o-xylene, 3-ethyltoluene, and 1,2,4-trimethylbenzene into intermediates of the citric acid cycle. With a monomer size of just 62 amino acid residues, the 4-Oxalocrotonate tautomerase is one of the smallest enzyme subunits known.[2] However, in solution, the enzyme forms a hexamer of six identical subunits, so the active site may be formed by amino acid residues from several subunits.[3] This enzyme is also unusual in that it uses a proline residue at the amino terminus as an active site residue.
References
- ^ Chen LH, Kenyon GL, Curtin F, Harayama S, Bembenek ME, Hajipour G, Whitman CP (September 1992). "4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer". The Journal of Biological Chemistry. 267 (25): 17716–21. PMID 1339435.
- ^ Whitman CP (June 2002). "The 4-oxalocrotonate tautomerase family of enzymes: how nature makes new enzymes using a beta-alpha-beta structural motif". Archives of Biochemistry and Biophysics. 402 (1): 1–13. doi:10.1016/S0003-9861(02)00052-8. PMID 12051677.
- ^ Subramanya HS, Roper DI, Dauter Z, Dodson EJ, Davies GJ, Wilson KS, Wigley DB (January 1996). "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases". Biochemistry. 35 (3): 792–802. doi:10.1021/bi951732k. PMID 8547259.
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Tautomerase enzyme Provide feedback
No Pfam abstract.
Internal database links
SCOOP: | CHMI DUF1904 Tautomerase Tautomerase_3 |
Similarity to PfamA using HHSearch: | Tautomerase |
This tab holds annotation information from the InterPro database.
InterPro entry IPR037479
Members of the tautomerase superfamily (TSF) are characterised by a beta-alpha-beta building block and a catalytic amino terminal proline [ PUBMED:12051677 , PUBMED:15381403 ]. There are five known TSF families, which are named for the first characterised member [ PUBMED:25219626 ].
This entry represents the malonate semialdehyde decarboxylase (MSAD) family [ PUBMED:14506256 ]. MSAD is part of a bacterial pathway for the degradation of the soil fumigant known as 1,3-dichloropropene [ PUBMED:14506256 ]. There are five homologues identified, including YusQ/YodA/YrdN from Bacillus subtilis, IolK from Lactobacillus casei strain BL23, and Bp4401 from Burkholderia phymatum. IolK was found in a degradative pathway for myo-inositol, but its disruption does not affect growth or myo-inositol utilization [ PUBMED:17449687 ]. Bp4401 has a modest hydratase activity. YrdN, YodA, and IolK have comparable decarboxylase and hydratase activities, but this activity is missing in YusQ and Bp4401 [ PUBMED:25219626 ].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan MIF (CL0082), which has the following description:
This clan groups 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI) and 4-oxalocrotonate tautomerase (4-OT) with macrophage inhibitory factor (MIF). Interestingly they all share an amino-terminal proline. Members of this clan for homotrimers [1].
The clan contains the following 6 members:
CHMI DUF1904 MIF Tautomerase Tautomerase_2 Tautomerase_3Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (68) |
Full (1495) |
Representative proteomes | UniProt (7433) |
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RP15 (133) |
RP35 (559) |
RP55 (1484) |
RP75 (3010) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (68) |
Full (1495) |
Representative proteomes | UniProt (7433) |
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RP15 (133) |
RP35 (559) |
RP55 (1484) |
RP75 (3010) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
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Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | CATH:3c6vA00, Pfam-B_819 (release 26.0) |
Previous IDs: | none |
Type: | Domain |
Sequence Ontology: | SO:0000417 |
Author: |
Coggill P |
Number in seed: | 68 |
Number in full: | 1495 |
Average length of the domain: | 79.70 aa |
Average identity of full alignment: | 30 % |
Average coverage of the sequence by the domain: | 60.70 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 82 | ||||||||||||
Family (HMM) version: | 8 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Tautomerase_2 domain has been found. There are 57 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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