Summary: Sensor_kinase_SpoOB-type, alpha-helical domain
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Sensor_kinase_SpoOB-type, alpha-helical domain Provide feedback
Sporulation initiation phospho-transferase B or SpoOB is part of a phospho-relay that initiates sporulation in Bacillus subtilis. Spo0B is a two-domain protein consisting of an N-terminal alpha-helical hairpin domain and a C-terminal alpha/beta domain. Two subunits of Spo0B dimerise by a parallel association of helical hairpins to form a novel four-helix bundle from which the active histidine - involved in the auto-phosphorylation - protrudes. In the phospho-relay, the signal-receptor histidine kinases are dephosphorylated by a common response regulator, Spo0F. Spo0B then takes phosphorylated Spo0F as substrate thereby mediating the transfer of a phosphoryl group to Spo0A, the ultimate transcription factor. The exact function of this alpha-helical domain is not known; it does not always occur just as the N-terminal domain of SPOB_ab, PF14682. SCOP describes this domain as a histidine kinase-like fold lacking the kinase ATP-binding site.
Varughese KI, Madhusudan, Zhou XZ, Whiteley JM, Hoch JA;, Mol Cell. 1998;2:485-493.: Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase. PUBMED:9809070 EPMC:9809070
Internal database links
|SCOOP:||Clathrin ECSIT Rep_fac_C RPM2 MRP-S27|
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This family is new in this Pfam release.
|Number in seed:||56|
|Number in full:||2392|
|Average length of the domain:||60.90 aa|
|Average identity of full alignment:||32 %|
|Average coverage of the sequence by the domain:||12.52 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||1|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the SPOB_a domain has been found. There are 10 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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