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0  structures 44  species 0  interactions 124  sequences 6  architectures

Family: Hce2 (PF14856)

Summary: Pathogen effector; putative necrosis-inducing factor

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Pathogen effector; putative necrosis-inducing factor Provide feedback

The domain corresponds to the mature part of the Ecp2 effector protein from the tomato pathogen Cladopsorium fulvum. Effectors are low molecular weight proteins that are secreted by bacteria, oomycetes and fungi to manipulate their hosts and adapt to their environment. Ecp2 is a 165 amino acid secreted protein that was originally identified as a virulence factor in C. fulvum, since disruption reduces virulence of the fungus on tomato plants. We have recently determined that Ecp2 is a member of a novel, widely distributed and highly diversified within the fungal kingdom multigene superfamily, which we have designated Hce2, for Homologs of C. fulvum Ecp2 effector. Although Ecp2 is present in most organisms as a small secreted protein, the mature part of this protein can be found fused to other protein domains, including the fungal Glycoside Hydrolase family 18, Glyco_hydro_18 PF00704 and other, unknown, protein domains. The intrinsic function of Ecp2 remains unknown but it is postulated by [3] that it is a necrosis-inducing factor in plants that serves pathogenicity on the host.

Literature references

  1. Van den Ackerveken GF, Van Kan JA, Joosten MH, Muisers JM, Verbakel HM, De Wit PJ;, Mol Plant Microbe Interact. 1993;6:210-215.: Characterization of two putative pathogenicity genes of the fungal tomato pathogen Cladosporium fulvum. PUBMED:8471794 EPMC:8471794

  2. Lauge R, Joosten MH, Haanstra JP, Goodwin PH, Lindhout P, De Wit PJ;, Proc Natl Acad Sci U S A. 1998;95:9014-9018.: Successful search for a resistance gene in tomato targeted against a virulence factor of a fungal pathogen. PUBMED:9671796 EPMC:9671796

  3. Stergiopoulos I, van den Burg HA, Okmen B, Beenen HG, van Liere S, Kema GH, de Wit PJ;, Proc Natl Acad Sci U S A. 2010;107:7610-7615.: Tomato Cf resistance proteins mediate recognition of cognate homologous effectors from fungi pathogenic on dicots and monocots. PUBMED:20368413 EPMC:20368413


External database links

This tab holds annotation information from the InterPro database.

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(39)
Full
(124)
Representative proteomes NCBI
(117)
Meta
(0)
RP15
(17)
RP35
(47)
RP55
(73)
RP75
(97)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(39)
Full
(124)
Representative proteomes NCBI
(117)
Meta
(0)
RP15
(17)
RP35
(47)
RP55
(73)
RP75
(97)
Alignment:
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Sequence:
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  Seed
(39)
Full
(124)
Representative proteomes NCBI
(117)
Meta
(0)
RP15
(17)
RP35
(47)
RP55
(73)
RP75
(97)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

This family is new in this Pfam release.

Seed source: [1]
Previous IDs: Ecp2;
Type: Family
Author: Stergiopoulos I, Coggill P
Number in seed: 39
Number in full: 124
Average length of the domain: 102.60 aa
Average identity of full alignment: 27 %
Average coverage of the sequence by the domain: 20.68 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.6 25.1
Noise cut-off 24.8 23.8
Model length: 96
Family (HMM) version: 1
Download: download the raw HMM for this family

Species distribution

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